Figure 3
PPC-SRµCT slice imaging and histological section. (a) Histological section of the PPTI sample with Saf-O/fast green staining and (b) a magnified image. (c) Original slice of the PPC-SRµCT reconstruction image. (d) Phase-contrast enhancement image of the PPTI. (e) 3D color-coding map of the PPCI slice after phase retrieval. The PPTI could be divided into four distinct zones along the longitudinal direction, including bone (zone I), calcified fibrocartilage (zone II), uncalcified fibrocartilage (zone III) and tendon (zone IV). The chondrocyte organization inside the PPTI was marked with a red rectangle. Scale bar = 800 µm (a); 200 µm (b and c); 250 µm (d). |