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Figure 2
Photographs of RG-lyase crystals. (a) Needle clusters, dendrites of RG-lyase from 26% PEG 4000, 0.3 M (NH4)2SO4, 0.1 M sodium acetate pH 4.5. (b) Spherulites from 24% PEG 4000, 0.1 M (NH4)2SO4, 0.1 M sodium acetate pH 4.8. (c) Stacked plates from 24% PEG 4000, 0.1 M (NH4)2SO4 and 0.1 M sodium acetate pH 4.4 at 277 K. (d) Single crystals from microseeding using a tenfold diluted seed stock with the condition 22% PEG 4000, 0.1 M (NH4)2SO4 and 0.1 M sodium acetate pH 4.4 at 277 K. (e) Larger crystals obtained by further dilution of seed stock, protein and precipitant concentration. (f) Single crystals from the condition 20% PEG 4000, 9% PEG 400, 0.1 M (NH4)2SO4 and 0.1 M sodium acetate pH 4.4 at 277 K.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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