 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 1]](pu0076fig1mag.jpg)
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Figure 1
Reaction and secondary structure of the hairpin ribozyme. (a) The general acid/base mechanism of the hairpin ribozyme. (b) Schematic depiction of the minimal hairpin ribozyme constructs used in this study; conserved residues are in bold type. Blunt as well as overhanging or `sticky' ends (black boxes) were screened in crystallization experiments. Dashed lines joining nucleotides 14 to 15 and 30.1 to 31 represent the phosphodiester linkage of junctioned constructs and a stem-loop, respectively; both features are absent in the ribozyme of this study. An arrow indicates the site of phosphodiester bond cleavage. Vertical bars are Watson–Crick hydrogen bonds. A 2′-O-methyl group has been incorporated at position A−1 to prevent bond cleavage. A black dot represents the site of 5-iodouracil incorporation for form II crystals. |
![[Figure 1]](pu0076fig1.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
