Figure 4
Close-up views of the antigen-combining site of the NNA7 and NNA7-G91S structures. (a) Surface representation of the antigen-combining site of the NNA7 Fab fragment complexed with MES (ball-and-stick model). The L chain is pink and the H chain is blue. The position of the L-chain G91S mutation is shown as a red patch. Simulated-annealing omit (Fo − Fc) electron-density map for the NNA7 MES molecule is shown at the 3σ contour level. (b) Schematic diagram of the mode of MES binding to NNA7. (c) A theoretical model of galactose binding to the same site as in (b). All amino-acid residues are positioned exactly as in the experimental NNA7 structure. Dashed lines indicate possible hydrogen bonds with associated distances; the red spheres indicate water molecules. |