Recombinant protein expression and solubility screening in Escherichia coli: a comparative study

Berrow, N.S.; Büssow, K.; Coutard, B.; Diprose, J.; Ekberg, M.; Folkers, G.E.; Levy, N.; Lieu, V.; Owens, R.J.; Peleg, Y.; Pinaglia, C.; Quevillon-Cheruel, S.; Salim, L.; Scheich, C.; Vincentelli, R.; Busso, D.

doi:10.1107/S0907444906031337

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 3
Effect of E. coli strains, temperature at induction and media on protein solubility. Summary of the results obtained by Marseille. The results of the sparse-matrix screen for soluble expression of the benchmark vectors are shown. The solubility level scores (0, 1, 2, 3) have been summed to give a total value for each strain/post-induction growth temperature/media combination and are indicated at the top of each bar.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 62| Part 10| September 2006| Pages 1218-1226

doi:10.1107/S0907444906031337

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