Eukaryotic expression: developments for structural proteomics

Aricescu, A.R.; Assenberg, R.; Bill, R.M.; Busso, D.; Chang, V.T.; Davis, S.J.; Dubrovsky, A.; Gustafsson, L.; Hedfalk, K.; Heinemann, U.; Jones, I.M.; Ksiazek, D.; Lang, C.; Maskos, K.; Messerschmidt, A.; Macieira, S.; Peleg, Y.; Perrakis, A.; Poterszman, A.; Schneider, G.; Sixma, T.K.; Sussman, J.L.; Sutton, G.; Tarboureich, N.; Zeev-Ben-Mordehai, T.; Jones, E.Y.

doi:10.1107/S0907444906029805

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

IUCr IT WDC

home archive editors for authors for readers submit subscribe open access

view article

Figure 4
Deglycosylation of the receptor tyrosine phosphatase RPTPμ expressed transiently in 293T and 293S/GnT1^−/− cells. 5 mg of purified protein was treated with 250 U of endoglycosidase (EndoH) at pH 5.2 for 6 h at 310 K in each case. The samples were then analysed by SDS–PAGE under reducing conditions; the band marked with an asterisk is EndoH. Expression of RPTPμ in 293S/GnT1^−/− cells leads to a larger fraction of the protein being `nicked'. In contrast to the partial EndoH-sensitivity of the 293T-derived material, the 293S/GnT1^−/−-derived protein is completely EndoH-sensitive.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 62| Part 10| September 2006| Pages 1114-1124

doi:10.1107/S0907444906029805

Follow Acta Cryst. D

Search IUCr Journals		doi		Advanced search
Author		volume	page