Structures and molecular-dynamics studies of three active-site mutants of bovine pancreatic phospholipase A₂

X-ray crystal structures have been obtained and molecular-dynamics simulations have been carried out for three active-site mutants (H48N, D49N and D49K) of bovine pancreatic phospholipase A₂. The active site is disturbed in the Asp49 mutant structures.

Observation of a calcium-binding site in the γ-class carbonic anhydrase from Pyrococcus horikoshii

Three crystal structures of the γ-carbonic anhydrase enzyme from P. horikoshii were solved in space group F4₁32. The enzyme forms a β-helix and each trimer is stacked in a tetrahedral manner in the crystal.

Systematic study on crystal-contact engineering of diphthine synthase: influence of mutations at crystal-packing regions on X-ray diffraction quality

The influence of mutations in crystal-packing regions on X-ray diffraction quality was systematically examined.

Structures of pseudechetoxin and pseudecin, two snake-venom cysteine-rich secretory proteins that target cyclic nucleotide-gated ion channels: implications for movement of the C-terminal cysteine-rich domain

The structures of pseudechetoxin and pseudecin suggest that both proteins bind to cyclic nucleotide-gated ion channels in a manner in which the concave surface occludes the pore entrance.

Nucleotide-binding flexibility in ultrahigh-resolution structures of the SRP GTPase Ffh

Crystal structures of the Ffh NG GTPase domain at < 1.24 Å resolution reveal multiple overlapping nucleotide binding modes.

Crystallization of proteins on functionalized surfaces

The influence of surfaces exposing ionizable groups on the crystallization of model proteins has been investigated.

Structure of an isolated unglycosylated antibody C_H2 domain

The crystal structure of an isolated unglycosylated antibody C_H2 domain has been determined at 1.7 Å resolution.

Structure of a haloacid dehalogenase superfamily phosphatase PH1421 from Pyrococcus horikoshii OT3: oligomeric state and thermoadaptation mechanism

The crystal structure of PH1421, a phosphatase belonging to the haloacid dehalogenase superfamily, has been determined at 1.6 Å resolution. The structure reveals a dimeric state which may be important for the catalytic function of the enzyme.

Patterson-guided ab initio analysis of structures with helical symmetry

Peaks in the Patterson maps help in finding molecular positions in the unit cell and assist in molecular-replacement solution in difficult cases for molecules with helical pseudosymmetry.