Oxidative folding of SOTI-III variants and inhibition curves of bovine pancreatic trypsin. (a) HPLC traces of wild-type linear precursor (1) and folded knottin (2) at 220 nm. (b) HPLC traces of F14A linear precursor (3) and folded knottin (4) at 220 nm. (c) The inhibition curves of SOTI-III (triangles) and SOTI-III F14A (squares) were obtained by fitting data to (1), as described in §2. Error bars were calculated from three independent experiments.