
Figure 4
Initial rate kinetics of SPy1599 on pNPGlc6P. (a) A MichaelisMenten fit of the initial rate kinetics on pNPGlc6P. Error bars are two standard deviations; the 95% confidence interval of the fitted curve is shown as dotted lines. (b) The pHrate profile of SPy1599 on pNPGlc6P, showing the effect of pH on SPy1599catalysed hydrolysis of 1 mM pNPGlc6P between pH 3.0 and 8.0. Filled black circles, sodium citrate buffer; filled black squares, MOPS buffer. Error bars are two standard deviations, representing a 95% confidence interval. The 95% confidence interval of the fitted curve is shown as dotted lines. 