Figure 4
Initial rate kinetics of SPy1599 on pNP-Glc6P. (a) A Michaelis-Menten fit of the initial rate kinetics on pNP-Glc6P. Error bars are two standard deviations; the 95% confidence interval of the fitted curve is shown as dotted lines. (b) The pH-rate profile of SPy1599 on pNP-Glc6P, showing the effect of pH on SPy1599-catalysed hydrolysis of 1 mM pNP-Glc6P between pH 3.0 and 8.0. Filled black circles, sodium citrate buffer; filled black squares, MOPS buffer. Error bars are two standard deviations, representing a 95% confidence interval. The 95% confidence interval of the fitted curve is shown as dotted lines.  [article HTML]

© International Union of Crystallography 2013