Comparison of E317 epitope and receptor-binding sites. (a) Surface representation of HSV-2 gD with residues involved in E317-Fab binding highlighted in orange. (b) The receptor-binding sites on HSV-1 gD corresponding to HSV-2 gD are highlighted. The HVEM-binding sites are shown in green and yellow (residues 25–32). The residues involved in nectin-1 binding are coloured pink and yellow. Both Gln27 and Leu28 make contact with two receptors. (c) The HSV-1 gD–HVEM complex structure (PDB entry 1jma
) was superimposed onto the HSV-2 gD structure; the N-terminal region of HSV-1 gD and HVEM are shown as green and magenta ribbons, respectively. E317 epitopes and HVEM-binding sites are shown as in the above figures. (d) Comparison with the interactions in gD–nectin-1. The key contact residues in the gD–nectin-1 interface are displayed as sticks and are labelled. The C atoms of HSV-1 gD and nectin-1 are coloured pink and blue, respectively. E317 and HSV-2 gD are shown as in Fig. 3(b).