Insights into the mechanism of X-ray-induced disulfide-bond cleavage in lysozyme crystals based on EPR, optical absorption and X-ray diffraction studies

Sutton, K.A.; Black, P.J.; Mercer, K.R.; Garman, E.F.; Owen, R.L.; Snell, E.H.; Bernhard, W.A.

doi:10.1107/S0907444913022117

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

IUCr IT WDC

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Figure 5
The dose response for radical trapping in lysozyme crystals irradiated with 70 keV X-rays at 4 K. Data for the concentration of total trapped radicals, R(tot), are shown for three different crystals using open black symbols (left y axis). Data for radicals formed by reduction of RSSR, R(SS), are shown using closed blue symbols (right y axis). See text regarding the normalization of the data using the measured mass of crystal 1. The curves were obtained by a nonlinear least-squares fit of the data using equations (6)