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Figure 5
Comparison of the tertiary structure of Psy-Lig with that of ChlV-Lig. (a) Superposition of Psy-Lig chain A (purple) with ChlV-Lig–AMP (green, PDB entry 1fvi) and ChlV-Lig–DNA (yellow, PDB entry 2q2t). The DNA-binding latch of ChlV-Lig and the homologous loop of Psy-Lig are coloured red. The unstructured residues of ChlV-Lig–AMP are indicated as a dashed line. DNA is shown in grey. (b) Psy-Lig modelled in the DNA-bound conformation by superposition of the Psy-Lig OB domain with the ChlV-Lig–DNA OB domain–DNA complex. The original adenylated Psy-Lig structure (purple) and the re-oriented position (pink) are shown as ribbons. The DNA from the ChlV-Lig–DNA structure is shown in grey. (c) Surface of the Psy-Lig DNA-bound model (pink) superposed with the ChlV-Lig–DNA structure (yellow, transparent), showing incomplete encirclement of the DNA by Psy-Lig.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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