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Figure 4
Paramagnetic labels attached to the FnIII-3 and FnIII-4 domains of β4. (a) The predicted average locations of the nitroxide group of MTSL attached to Cys are shown as spheres in the crystal structure of FnIII-3. The positions that yielded well defined inter-spin distances with a second probe at C1608 are coloured green. Those that resulted in broad distance distributions but did not alter the global structure of FnIII-3,4 are coloured magenta. The positions that resulted in a distortion of the structure are coloured dark violet. (b) Crystal structure of FnIII-4 with the estimated average position of the paramagnetic groups. The positions that yielded useful distances are coloured green, while those that resulted in broad distributions are shown in pink.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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