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Figure 3
Multi-crystal data collection and structure solution from microcrystals of bacteriorhodopsin. (a) Microcrystals of bacteriorhodopsin obtained from crystallization in lipidic mesophase; average crystal size ∼5 × 5 × 2 µm. (b) Heat map after initial mesh scan of the sample holder. (c) Dendrogram based on HCA of CCI(i, j) values produced by XSCALE. (d) Wilson plot from the final data set derived using BEST. (e) Detail of the final 2mFobsDFcalc, αcalc electron-density map (contoured at 1.5 × r.m.s.), with the refined structure shown in ball-and-stick representation. (f) OMIT difference density (mFobsDFcalc, αcalc) map at the end of the refinement procedure (contoured at 2.0 × r.m.s.) for a retinal molecule (ball-and-stick representation).

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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