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Figure 3
(a) Schematic representation of the high-speed spectroscopy setup for mapping the loaded chip. (b) Example spectrum of a carboxymyoglobin crystal recorded from a crystal-containing feature. The characteristic absorption bands for carboxymyoglobin (542 and 579 nm; Springer & Sligar, 1987BB38) are indicated. (c) The chip mounted in the beam at the XPP instrument at LCLS. (d) A magnified picture of chip compartments loaded with crystals. The carboxymyoglobin crystals can be easily identified owing to their red colour. (e) Three-dimensional image of crystals trapped inside a feature (image taken using a Hirox RH2000 microscope as part of user facilities on site at SACLA, RIKEN, Japan).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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