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Figure 3
Construct design of the PDE5/PDE4 chimera protein. (a) Domain boundaries of the original PDE5A1 catalytic domain construct with disordered residues highlighted in blue and the proteolytic sites marked. (b) Sequence alignment between the loop region of PDE5A1 (top row) with equivalent residues in PDE4B2B (bottom row) and the sequence of the PDE5A1/PDE4B2B chimera protein (middle row). (c) Cartoon representation of the crystal structure of PDE4B2B (PDB entry 4nw7) with the loop insertion region highlighted in orange, created using CCP4mg molecular-graphics software (McNicholas et al., 2011BB39).

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ISSN: 2059-7983
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