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Figure 2
Overview of the parts and actions required before high-pressure cooling. (a) The sample unit consists of a polyimide capillary, a copper pin and a pin holder. (b) The crystal is harvested from the mother liquor with the capillary. (c) The capillary is filled with solution and crystal and sealed with saturated PEG 100 000 solution. (d) The sample unit with solution and crystal is inserted into the cartridge. This takes place in the loading station of the high-pressure cooler and is guided by a loading groove and a cylindrical tool.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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