forthcoming articles

Solution structures of complexes between human serum albumin and the two lipidated insulin analogues, detemir and degludec are presented, and models for the equilibria are suggested.

A wide variety of neutron scattering techniques can be applied to obtain information on the structure and dynamics of biological systems on multiple scales. Recent progress is summarized, as are hoped-for future developments in the context of the advent of next-generation neutron sources on various continents.

Abstract The heterododecameric complex of a reducing octaheme cytochrome c with its redox partner shows an extended electron transfer pathway.

Catabolism of fatty acids in mycobacteria involves an extensively redundant set of enzymes. Here describe the structure of L-3-hy­droxy­acyl-CoA de­hydrogenase FadB2, and explore structural cues as to how it may work with other components of β-oxidation.

The N-acetyl­glucosamine 2-epimerase (AGE) from Nostoc sp. KVJ10 (nAGE10) has been crystallized in a different space group than other AGEs. The nAGE10 dimer, while different from the proposed AGE dimer in previously published structures, can be found in those and is probably the biological one.

The surface-entropy reduction method has been used to generate new crystal forms of the human O-GlcNAcase.

Crystal structures of the complex of a serine protease inhibitor from B. bauhinioides with bovine trypsin were determined in two crystal forms, which served as templates for modeling the structures of its complexes with plasma and tissue kallikreins.

LexA, a protein that is involved in the SOS response, from M. tuberculosis and its mutants have been biochemically characterized and the structures of their catalytic segments have been determined.

Biological Small-Angle Scattering: Theory and Practice by Eaton E. Lattman, Thomas D. Grant and Edward H. Snell is reviewed.

Merging statistics are evaluated in multi crystal SAD and used to optimized the anomalous signal.

Current developments and challenges for serial sample delivery at synchrotrons and X-ray free-electron lasers are reviewed, including the new megahertz repetition-rate machines, with an emphasis on liquid injection and high-viscosity extrusion.

Guanine N7–Zn²⁺ coordination served as the basis for the determination of three DNA crystal structures using zinc single-wavelength anomalous diffraction. This method is likely to be broadly applicable to the experimental determination of crystal structures of nucleic acids.

The structure of interferon-stimulated gene product 15 (ISG15) from the bat species M. davidii was elucidated to 1.37 Å resolution. Structural comparison of this ISG15 originating from a species of vesper bat endemic to China with those from mouse and human illuminates a conserved hydrophobic interface between the ubiquitin-like domains of ISG15. The importance of this interaction was probed for its influence on ISG15 engagement by a papain-like viral deISGylase using isothermal titration calorimetry.

A set of restraints for an iron–sulfur cluster based on small-molecule structures was generated and tested in structure refinement. Additionally, the small-molecule structures also provided bond and angle restraints for linking the cluster to the coordinating cysteine residues.

The X-ray structure and SAXS analysis of the C-terminal domain of the nucleocapsid from Middle East respiratory syndrome coronavirus, an emerging virus, are reported.

In the light of NMR data on product profiles, the structure of an Alicyclobacillus sp. CAZy family GH13 α-amylase highlights the accommodation of branch points in the α-amylase active centre.

Here we present an automated method that diagnoses indexing ambiguities and produces a consistent indexing choice for the large majority of diffraction images.