forthcoming articles

Crystal structures of K. pneumoniae pullulanase (KPP) in complex with α-, β- or γ-cyclodextrin were determined at around 1.98–2.59 Å resolution. Cyclodextrins interact with CBM41 and the active site of KPP. In the active site, Phe746 interacts with the inner cavity of the cyclodextrin rings. The strong inhibition of KPP by β-cyclodextrin is caused by the optimized interaction between β-cyclodextrin and the side chain of Phe746. These structures and the kinetic parameters of recombinant pullulanase and the F746A variant revealed the importance of Phe746 in the selective binding of cyclodextrins.

The sixth edition of Biochemistry and Molecular Biology by edited by Despo Papachristodoulou, Alison Snape, William H. Elliott and Daphne C. Elliott is reviewed.

A detailed analysis of a winged helix-turn-helix family transcriptional regulator that positively regulates archaeal biofilm formation is reported.

The program Buccaneer has been integrated in a cyclic automatic model-building procedure. improving its efficiency and accuracy.

It is demonstrated that using three-dimensional profile fitting of Bragg peaks increases the accuracy and resolution of neutron crystallographic data collected from proteins and reveals new features in nuclear density maps calculated from these data.

Here we present an automated method that diagnoses indexing ambiguities and produces a consistent indexing choice for the large majority of diffraction images.

The crystal structure of α-L-rhamnosidase, a biotechnologically important enzyme isolated from A. terreus, reveals its active-site architecture, domain organization and native glycosylation.

Quantum-mechanics/molecular-mechanics (ONIOM) X-ray macromolecular refinement using the program DivCon integrated with PHENIX is reported.

The crystal structures of two fimbrial adhesins from the uropathogen P. mirabilis are reported as a first step towards unveiling their role in pathogenesis. Despite lacking significant sequence homology, the two structures are very similar.

A step-by-step guide to processing serial crystallography data using CrystFEL for XFELs and synchrotron sources.

In this article, the status of the STARGazer data-processing software and its data-processing algorithms are described in detail. The STARGazer data-processing software is used for neutron time-of-flight single-crystal diffraction data collected using the IBARAKI Biological Crystal Diffractometer.

Using neutron reflectivity, we provide a comprehensive structural characterization of each step in the formation of a protein-tethered bilayer lipid membrane. We report relevant physical parameters of self-assembled monolayers, surface-captured detergent-stabilized protein, and the bilayer lipid membrane reconstituted by dialysis around the surface-captured tethering proteins.

Multiple dose-dependent room-temperature structures of copper nitrite reductase were determined from microcrystals in a single silicon nitride fixed-target chip. The separation of crystal polymorphs into distinct structures is described, together with the characterization of X-ray-irradiation effects through the dose series.