Supporting information
Crystallographic Information File (CIF) https://doi.org/10.1107/S1600536803012571/ci6226sup1.cif | |
Structure factor file (CIF format) https://doi.org/10.1107/S1600536803012571/ci6226Isup2.hkl |
CCDC reference: 217468
Key indicators
- Single-crystal X-ray study
- T = 296 K
- Mean (C-C) = 0.007 Å
- R factor = 0.057
- wR factor = 0.158
- Data-to-parameter ratio = 10.1
checkCIF results
No syntax errors found ADDSYM reports no extra symmetry General Notes
REFLT_03 From the CIF: _diffrn_reflns_theta_max 26.00 From the CIF: _reflns_number_total 3203 Count of symmetry unique reflns 3209 Completeness (_total/calc) 99.81% TEST3: Check Friedels for noncentro structure Estimate of Friedel pairs measured 0 Fraction of Friedel pairs measured 0.000 Are heavy atom types Z>Si present no Please check that the estimate of the number of Friedel pairs is correct. If it is not, please give the correct count in the _publ_section_exptl_refinement section of the submitted CIF.
The rhizomes of Astilbe Chinensis were collected in Anji county, Zhejiang province, China in August 2001. The plants were identified as A. chinensis (Haxim.) Franch. et Savat. by Professor Xiang-Ji Xue, College of Pharmaceutical Science, Zhejiang University. A voucher specimen (No. 200120) was deposited in Laboratory of Nature and Biochemistry, Zhejiang University. The rhizomes of Astilbe Chinensis were dried at 313 K in the dark, in a ventilated hood, and ground. The material (5.0 kg) was extracted three times with CH3OH (each 25 l) at room temperature. The CH3OH extract (366 g) was partitioned between petroleum ether (20 l) and water (2 l). The petroleum ether solution was concentrated in vacuum to give 41.2 g of a gelatinous material. A part of the extract (40.0 g) was absorbed onto silica gel (60 g) and chromatographied on a silica gel (600 g) column eluted with petroleum ether-EtoAc (50:1, 30:1, 15:1, 5:1, 3:1, 2:1) gradients. The eluted fractions were evaluated by TLC and combined to give fractions 1 to 19, respectively. Fraction 12 was recrystallized with EtOAc–CH3OH (1:1) to afford 2.5 g of the pure title compound, (I). m.p.: 513.5–515.5 K. 13C NMR (125 MHz, C5ND5, p.p.m): 179.4 (C27), 138.2 (C13), 126.2 (C12), 79.4 (C3), 55.8 (C14), 55.2 (C5), 49.0 (C18), 47.2 (C9), 43.9 (C19), 39.7 (C8), 7 (C4), 38.6 (C1), 37.1 (C10), 36.5 (C21), 36.3 (C7), 34.3 (C22), 33.5 (C29), 38 32.8 (C17), 31.1 (C20), 28.2 (C28), 28.1 (C23), 27.5(C2),26.9 (C16), 23.6 (C30), 22.8 (C11), 22.2 (C15), 18.2 (C6), 18.0 (C26), 16.4 (C25), 16.0 (C24). Crystals suitable for X-ray structure analysis were obtained by slow evaporation from methanol at room temperature.
After checking their presence in the difference map, all H atoms were geometrically fixed and allowed to ride on their attached atoms using SHELXL97 (Sheldrick, 1997) defaults. The absolute structure could not be established because of the weak anomalous signal. Friedel pairs were merged for the final cycles of refinement.
Data collection: XSCANS (Siemens, 1994); cell refinement: XSCANS; data reduction: XSCANS; program(s) used to solve structure: SHELXS97 (Sheldrick, 1997); program(s) used to refine structure: SHELXL97 (Sheldrick, 1997); molecular graphics: SHELXTL/PC (Siemens, 1991); software used to prepare material for publication: SHELXL97.
Fig. 1. A view of (I), with the atomic numbering scheme. Displacement ellipsoids are drawn at the 35% probability level. H atoms have been omitted for clarity. |
C30H48O3·CH4O | F(000) = 1080 |
Mr = 488.73 | Dx = 1.137 Mg m−3 |
Orthorhombic, P212121 | Mo Kα radiation, λ = 0.71073 Å |
Hall symbol: P 2ac 2ab | Cell parameters from 38 reflections |
a = 7.538 (2) Å | θ = 2.9–13.8° |
b = 13.710 (3) Å | µ = 0.07 mm−1 |
c = 27.629 (8) Å | T = 296 K |
V = 2855.3 (13) Å3 | Prism, colorless |
Z = 4 | 0.58 × 0.40 × 0.24 mm |
Siemens P4 diffractometer | Rint = 0.021 |
Radiation source: normal-focus sealed tube | θmax = 26.0°, θmin = 1.7° |
Graphite monochromator | h = 0→9 |
ω scans | k = 0→16 |
3492 measured reflections | l = −1→34 |
3203 independent reflections | 3 standard reflections every 97 reflections |
1876 reflections with I > 2σ(I) | intensity decay: 1.5% |
Refinement on F2 | Secondary atom site location: difference Fourier map |
Least-squares matrix: full | Hydrogen site location: inferred from neighbouring sites |
R[F2 > 2σ(F2)] = 0.057 | H-atom parameters constrained |
wR(F2) = 0.158 | w = 1/[σ2(Fo2) + (0.0951P)2] where P = (Fo2 + 2Fc2)/3 |
S = 0.89 | (Δ/σ)max < 0.001 |
3203 reflections | Δρmax = 0.21 e Å−3 |
316 parameters | Δρmin = −0.19 e Å−3 |
0 restraints | Extinction correction: SHELXTL, Fc*=kFc[1+0.001xFc2λ3/sin(2θ)]-1/4 |
Primary atom site location: structure-invariant direct methods | Extinction coefficient: 0.0116 (17) |
C30H48O3·CH4O | V = 2855.3 (13) Å3 |
Mr = 488.73 | Z = 4 |
Orthorhombic, P212121 | Mo Kα radiation |
a = 7.538 (2) Å | µ = 0.07 mm−1 |
b = 13.710 (3) Å | T = 296 K |
c = 27.629 (8) Å | 0.58 × 0.40 × 0.24 mm |
Siemens P4 diffractometer | Rint = 0.021 |
3492 measured reflections | 3 standard reflections every 97 reflections |
3203 independent reflections | intensity decay: 1.5% |
1876 reflections with I > 2σ(I) |
R[F2 > 2σ(F2)] = 0.057 | 0 restraints |
wR(F2) = 0.158 | H-atom parameters constrained |
S = 0.89 | Δρmax = 0.21 e Å−3 |
3203 reflections | Δρmin = −0.19 e Å−3 |
316 parameters |
Geometry. All e.s.d.'s (except the e.s.d. in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell e.s.d.'s are taken into account individually in the estimation of e.s.d.'s in distances, angles and torsion angles; correlations between e.s.d.'s in cell parameters are only used when they are defined by crystal symmetry. An approximate (isotropic) treatment of cell e.s.d.'s is used for estimating e.s.d.'s involving l.s. planes. |
Refinement. Refinement of F2 against ALL reflections. The weighted R-factor wR and goodness of fit S are based on F2, conventional R-factors R are based on F, with F set to zero for negative F2. The threshold expression of F2 > σ(F2) is used only for calculating R-factors(gt) etc. and is not relevant to the choice of reflections for refinement. R-factors based on F2 are statistically about twice as large as those based on F, and R- factors based on ALL data will be even larger. |
x | y | z | Uiso*/Ueq | ||
O1 | 0.4762 (4) | −0.0208 (3) | 0.51890 (12) | 0.0620 (10) | |
H1 | 0.3817 | −0.0143 | 0.5333 | 0.093* | |
O2 | 0.4050 (4) | 0.5275 (2) | 0.37966 (11) | 0.0560 (9) | |
H2 | 0.3778 | 0.5625 | 0.4026 | 0.084* | |
O3 | 0.6369 (4) | 0.4965 (2) | 0.42622 (10) | 0.0484 (8) | |
C1 | 0.7680 (6) | 0.1705 (3) | 0.46233 (16) | 0.0449 (12) | |
H1A | 0.8964 | 0.1731 | 0.4603 | 0.054* | |
H1B | 0.7287 | 0.2264 | 0.4810 | 0.054* | |
C2 | 0.7130 (6) | 0.0764 (4) | 0.48897 (18) | 0.0491 (13) | |
H2A | 0.7607 | 0.0204 | 0.4719 | 0.059* | |
H2B | 0.7628 | 0.0768 | 0.5213 | 0.059* | |
C3 | 0.5152 (6) | 0.0675 (4) | 0.49213 (16) | 0.0473 (12) | |
H3 | 0.4715 | 0.1227 | 0.5112 | 0.057* | |
C4 | 0.4211 (6) | 0.0704 (3) | 0.44268 (17) | 0.0430 (11) | |
C5 | 0.4858 (5) | 0.1643 (3) | 0.41638 (15) | 0.0356 (10) | |
H5 | 0.4515 | 0.2170 | 0.4384 | 0.043* | |
C6 | 0.3910 (6) | 0.1891 (3) | 0.36983 (16) | 0.0445 (11) | |
H6A | 0.2652 | 0.1764 | 0.3734 | 0.053* | |
H6B | 0.4357 | 0.1483 | 0.3439 | 0.053* | |
C7 | 0.4200 (6) | 0.2978 (3) | 0.35689 (16) | 0.0413 (11) | |
H7A | 0.3531 | 0.3135 | 0.3279 | 0.050* | |
H7B | 0.3757 | 0.3383 | 0.3830 | 0.050* | |
C8 | 0.6169 (5) | 0.3207 (3) | 0.34835 (14) | 0.0341 (10) | |
C9 | 0.7282 (5) | 0.2852 (3) | 0.39223 (15) | 0.0346 (10) | |
H9 | 0.6953 | 0.3281 | 0.4191 | 0.042* | |
C10 | 0.6899 (5) | 0.1774 (3) | 0.41076 (16) | 0.0366 (11) | |
C11 | 0.9246 (6) | 0.3043 (3) | 0.38315 (17) | 0.0454 (12) | |
H11A | 0.9878 | 0.3004 | 0.4137 | 0.055* | |
H11B | 0.9708 | 0.2536 | 0.3621 | 0.055* | |
C12 | 0.9599 (6) | 0.4020 (3) | 0.36042 (16) | 0.0421 (11) | |
H12 | 1.0777 | 0.4221 | 0.3591 | 0.050* | |
C13 | 0.8396 (6) | 0.4630 (3) | 0.34187 (15) | 0.0378 (10) | |
C14 | 0.6421 (5) | 0.4355 (3) | 0.34280 (15) | 0.0371 (10) | |
C15 | 0.5490 (6) | 0.4746 (4) | 0.29624 (16) | 0.0466 (12) | |
H15A | 0.5726 | 0.4296 | 0.2699 | 0.056* | |
H15B | 0.4218 | 0.4749 | 0.3017 | 0.056* | |
C16 | 0.6056 (7) | 0.5765 (4) | 0.28055 (18) | 0.0549 (13) | |
H16A | 0.5695 | 0.6232 | 0.3050 | 0.066* | |
H16B | 0.5456 | 0.5932 | 0.2506 | 0.066* | |
C17 | 0.8069 (6) | 0.5839 (4) | 0.27293 (17) | 0.0511 (13) | |
C18 | 0.8975 (6) | 0.5597 (3) | 0.32132 (15) | 0.0422 (11) | |
H18 | 1.0243 | 0.5532 | 0.3142 | 0.051* | |
C19 | 0.8813 (7) | 0.6423 (3) | 0.35877 (16) | 0.0507 (12) | |
H19A | 0.7580 | 0.6480 | 0.3684 | 0.061* | |
H19B | 0.9490 | 0.6246 | 0.3873 | 0.061* | |
C20 | 0.9454 (8) | 0.7419 (4) | 0.3410 (2) | 0.0640 (15) | |
C21 | 0.8390 (8) | 0.7660 (4) | 0.2944 (2) | 0.0740 (18) | |
H21A | 0.7146 | 0.7734 | 0.3026 | 0.089* | |
H21B | 0.8803 | 0.8276 | 0.2813 | 0.089* | |
C22 | 0.8581 (8) | 0.6870 (4) | 0.25607 (18) | 0.0685 (16) | |
H22A | 0.9804 | 0.6859 | 0.2451 | 0.082* | |
H22B | 0.7848 | 0.7042 | 0.2285 | 0.082* | |
C23 | 0.4504 (7) | −0.0255 (4) | 0.41420 (18) | 0.0592 (14) | |
H23A | 0.3728 | −0.0751 | 0.4266 | 0.089* | |
H23B | 0.5714 | −0.0462 | 0.4178 | 0.089* | |
H23C | 0.4250 | −0.0148 | 0.3806 | 0.089* | |
C24 | 0.2222 (6) | 0.0786 (4) | 0.45259 (19) | 0.0564 (14) | |
H24A | 0.1871 | 0.0289 | 0.4751 | 0.085* | |
H24B | 0.1579 | 0.0706 | 0.4229 | 0.085* | |
H24C | 0.1965 | 0.1416 | 0.4660 | 0.085* | |
C25 | 0.7728 (6) | 0.0986 (3) | 0.37885 (18) | 0.0518 (13) | |
H25A | 0.8036 | 0.0432 | 0.3984 | 0.078* | |
H25B | 0.8776 | 0.1240 | 0.3636 | 0.078* | |
H25C | 0.6893 | 0.0791 | 0.3545 | 0.078* | |
C26 | 0.6791 (7) | 0.2719 (4) | 0.30065 (16) | 0.0508 (12) | |
H26A | 0.6879 | 0.2027 | 0.3053 | 0.076* | |
H26B | 0.7930 | 0.2975 | 0.2917 | 0.076* | |
H26C | 0.5949 | 0.2854 | 0.2755 | 0.076* | |
C27 | 0.5642 (6) | 0.4899 (3) | 0.38719 (16) | 0.0394 (11) | |
C28 | 0.8725 (8) | 0.5138 (4) | 0.23351 (15) | 0.0672 (16) | |
H28A | 0.8102 | 0.5266 | 0.2039 | 0.101* | |
H28B | 0.8510 | 0.4478 | 0.2434 | 0.101* | |
H28C | 0.9974 | 0.5232 | 0.2285 | 0.101* | |
C29 | 1.1465 (7) | 0.7437 (4) | 0.3318 (2) | 0.0716 (17) | |
H29A | 1.2082 | 0.7308 | 0.3615 | 0.107* | |
H29B | 1.1803 | 0.8067 | 0.3197 | 0.107* | |
H29C | 1.1765 | 0.6947 | 0.3083 | 0.107* | |
C30 | 0.9068 (11) | 0.8182 (4) | 0.3801 (2) | 0.103 (2) | |
H30A | 0.9762 | 0.8041 | 0.4084 | 0.154* | |
H30B | 0.7830 | 0.8165 | 0.3883 | 0.154* | |
H30C | 0.9370 | 0.8819 | 0.3683 | 0.154* | |
O4 | 0.2549 (5) | 0.6319 (3) | 0.44767 (13) | 0.0648 (10) | |
H4O | 0.1650 | 0.6089 | 0.4599 | 0.097* | |
C31 | 0.3446 (14) | 0.6856 (6) | 0.4815 (3) | 0.134 (3) | |
H31A | 0.4700 | 0.6785 | 0.4765 | 0.201* | |
H31B | 0.3144 | 0.6628 | 0.5133 | 0.201* | |
H31C | 0.3124 | 0.7530 | 0.4785 | 0.201* |
U11 | U22 | U33 | U12 | U13 | U23 | |
O1 | 0.042 (2) | 0.070 (2) | 0.074 (2) | −0.0003 (18) | 0.0055 (18) | 0.029 (2) |
O2 | 0.0364 (18) | 0.061 (2) | 0.070 (2) | 0.0182 (17) | 0.0001 (17) | 0.0005 (18) |
O3 | 0.0497 (19) | 0.052 (2) | 0.0433 (17) | 0.0130 (16) | 0.0019 (16) | 0.0015 (16) |
C1 | 0.028 (2) | 0.046 (3) | 0.061 (3) | 0.000 (2) | −0.007 (2) | 0.011 (2) |
C2 | 0.036 (3) | 0.049 (3) | 0.062 (3) | 0.001 (2) | −0.008 (2) | 0.015 (3) |
C3 | 0.043 (3) | 0.053 (3) | 0.046 (3) | 0.005 (2) | 0.002 (2) | 0.014 (3) |
C4 | 0.032 (2) | 0.038 (2) | 0.059 (3) | 0.000 (2) | 0.003 (2) | 0.004 (2) |
C5 | 0.028 (2) | 0.037 (2) | 0.041 (2) | 0.0009 (19) | −0.0053 (19) | 0.001 (2) |
C6 | 0.034 (2) | 0.048 (3) | 0.052 (3) | −0.003 (2) | −0.007 (2) | 0.003 (2) |
C7 | 0.032 (2) | 0.044 (3) | 0.048 (3) | 0.003 (2) | −0.008 (2) | 0.005 (2) |
C8 | 0.029 (2) | 0.034 (2) | 0.039 (2) | 0.0038 (19) | −0.0023 (19) | −0.0006 (19) |
C9 | 0.029 (2) | 0.030 (2) | 0.046 (2) | 0.0039 (19) | −0.004 (2) | 0.0005 (19) |
C10 | 0.028 (2) | 0.029 (2) | 0.053 (3) | 0.0028 (19) | −0.001 (2) | 0.000 (2) |
C11 | 0.032 (2) | 0.041 (3) | 0.063 (3) | 0.001 (2) | −0.005 (2) | 0.011 (2) |
C12 | 0.028 (2) | 0.044 (3) | 0.054 (3) | 0.007 (2) | 0.001 (2) | 0.008 (2) |
C13 | 0.031 (2) | 0.048 (3) | 0.035 (2) | 0.003 (2) | 0.001 (2) | 0.001 (2) |
C14 | 0.028 (2) | 0.051 (3) | 0.032 (2) | 0.005 (2) | 0.0012 (19) | 0.005 (2) |
C15 | 0.038 (3) | 0.052 (3) | 0.050 (3) | 0.001 (2) | −0.010 (2) | 0.009 (2) |
C16 | 0.055 (3) | 0.064 (3) | 0.045 (3) | 0.004 (3) | −0.013 (3) | 0.016 (3) |
C17 | 0.046 (3) | 0.062 (3) | 0.046 (3) | 0.000 (3) | 0.000 (2) | 0.015 (3) |
C18 | 0.034 (2) | 0.048 (3) | 0.045 (2) | 0.007 (2) | 0.000 (2) | 0.005 (2) |
C19 | 0.054 (3) | 0.047 (3) | 0.052 (3) | 0.003 (3) | 0.008 (3) | 0.008 (2) |
C20 | 0.077 (4) | 0.047 (3) | 0.068 (3) | −0.001 (3) | 0.003 (3) | 0.009 (3) |
C21 | 0.068 (4) | 0.052 (3) | 0.102 (5) | 0.000 (3) | −0.006 (4) | 0.035 (3) |
C22 | 0.076 (4) | 0.067 (4) | 0.062 (3) | 0.002 (3) | −0.006 (3) | 0.026 (3) |
C23 | 0.053 (3) | 0.044 (3) | 0.081 (4) | −0.006 (3) | 0.003 (3) | 0.000 (3) |
C24 | 0.036 (3) | 0.065 (3) | 0.068 (3) | −0.002 (3) | 0.001 (3) | 0.013 (3) |
C25 | 0.041 (3) | 0.043 (3) | 0.072 (3) | 0.010 (2) | 0.011 (3) | 0.000 (3) |
C26 | 0.046 (3) | 0.058 (3) | 0.048 (3) | −0.001 (3) | 0.003 (2) | −0.003 (2) |
C27 | 0.036 (3) | 0.038 (3) | 0.044 (3) | 0.004 (2) | −0.001 (2) | 0.009 (2) |
C28 | 0.083 (4) | 0.078 (4) | 0.040 (3) | −0.006 (4) | 0.009 (3) | 0.001 (3) |
C29 | 0.066 (4) | 0.072 (4) | 0.077 (4) | −0.015 (3) | −0.008 (3) | 0.019 (3) |
C30 | 0.144 (7) | 0.048 (3) | 0.115 (5) | −0.002 (4) | 0.025 (6) | −0.010 (4) |
O4 | 0.059 (2) | 0.061 (2) | 0.075 (2) | 0.008 (2) | 0.017 (2) | 0.009 (2) |
C31 | 0.169 (9) | 0.127 (7) | 0.104 (5) | −0.023 (7) | −0.015 (6) | −0.023 (5) |
O1—C3 | 1.449 (5) | C16—C17 | 1.536 (7) |
O1—H1 | 0.82 | C16—H16A | 0.97 |
O2—C27 | 1.323 (5) | C16—H16B | 0.97 |
O2—H2 | 0.82 | C17—C28 | 1.534 (7) |
O3—C27 | 1.213 (5) | C17—C22 | 1.537 (7) |
C1—C2 | 1.542 (6) | C17—C18 | 1.538 (6) |
C1—C10 | 1.544 (6) | C18—C19 | 1.540 (6) |
C1—H1A | 0.97 | C18—H18 | 0.98 |
C1—H1B | 0.97 | C19—C20 | 1.530 (7) |
C2—C3 | 1.499 (6) | C19—H19A | 0.97 |
C2—H2A | 0.97 | C19—H19B | 0.97 |
C2—H2B | 0.97 | C20—C30 | 1.534 (8) |
C3—C4 | 1.540 (6) | C20—C29 | 1.537 (8) |
C3—H3 | 0.98 | C20—C21 | 1.552 (7) |
C4—C24 | 1.528 (6) | C21—C22 | 1.522 (8) |
C4—C23 | 1.549 (6) | C21—H21A | 0.97 |
C4—C5 | 1.556 (6) | C21—H21B | 0.97 |
C5—C6 | 1.510 (6) | C22—H22A | 0.97 |
C5—C10 | 1.556 (6) | C22—H22B | 0.97 |
C5—H5 | 0.98 | C23—H23A | 0.96 |
C6—C7 | 1.548 (6) | C23—H23B | 0.96 |
C6—H6A | 0.97 | C23—H23C | 0.96 |
C6—H6B | 0.97 | C24—H24A | 0.96 |
C7—C8 | 1.535 (6) | C24—H24B | 0.96 |
C7—H7A | 0.97 | C24—H24C | 0.96 |
C7—H7B | 0.97 | C25—H25A | 0.96 |
C8—C26 | 1.550 (6) | C25—H25B | 0.96 |
C8—C9 | 1.552 (5) | C25—H25C | 0.96 |
C8—C14 | 1.593 (6) | C26—H26A | 0.96 |
C9—C11 | 1.524 (6) | C26—H26B | 0.96 |
C9—C10 | 1.591 (5) | C26—H26C | 0.96 |
C9—H9 | 0.98 | C28—H28A | 0.96 |
C10—C25 | 1.528 (6) | C28—H28B | 0.96 |
C11—C12 | 1.503 (6) | C28—H28C | 0.96 |
C11—H11A | 0.97 | C29—H29A | 0.96 |
C11—H11B | 0.97 | C29—H29B | 0.96 |
C12—C13 | 1.336 (6) | C29—H29C | 0.96 |
C12—H12 | 0.93 | C30—H30A | 0.96 |
C13—C18 | 1.506 (6) | C30—H30B | 0.96 |
C13—C14 | 1.536 (6) | C30—H30C | 0.96 |
C14—C27 | 1.551 (6) | O4—C31 | 1.369 (8) |
C14—C15 | 1.561 (6) | O4—H4O | 0.82 |
C15—C16 | 1.524 (7) | C31—H31A | 0.96 |
C15—H15A | 0.97 | C31—H31B | 0.96 |
C15—H15B | 0.97 | C31—H31C | 0.96 |
C3—O1—H1 | 109.5 | C17—C16—H16B | 109.2 |
C27—O2—H2 | 109.5 | H16A—C16—H16B | 107.9 |
C2—C1—C10 | 112.9 (4) | C28—C17—C16 | 112.0 (5) |
C2—C1—H1A | 109.0 | C28—C17—C22 | 106.2 (4) |
C10—C1—H1A | 109.0 | C16—C17—C22 | 110.5 (4) |
C2—C1—H1B | 109.0 | C28—C17—C18 | 109.8 (4) |
C10—C1—H1B | 109.0 | C16—C17—C18 | 107.8 (4) |
H1A—C1—H1B | 107.8 | C22—C17—C18 | 110.5 (4) |
C3—C2—C1 | 111.3 (4) | C13—C18—C17 | 112.9 (4) |
C3—C2—H2A | 109.4 | C13—C18—C19 | 111.8 (3) |
C1—C2—H2A | 109.4 | C17—C18—C19 | 113.0 (4) |
C3—C2—H2B | 109.4 | C13—C18—H18 | 106.2 |
C1—C2—H2B | 109.4 | C17—C18—H18 | 106.2 |
H2A—C2—H2B | 108.0 | C19—C18—H18 | 106.2 |
O1—C3—C2 | 107.5 (4) | C20—C19—C18 | 114.6 (4) |
O1—C3—C4 | 112.4 (4) | C20—C19—H19A | 108.6 |
C2—C3—C4 | 113.9 (4) | C18—C19—H19A | 108.6 |
O1—C3—H3 | 107.6 | C20—C19—H19B | 108.6 |
C2—C3—H3 | 107.6 | C18—C19—H19B | 108.6 |
C4—C3—H3 | 107.6 | H19A—C19—H19B | 107.6 |
C24—C4—C3 | 107.2 (4) | C19—C20—C30 | 108.8 (4) |
C24—C4—C23 | 107.1 (4) | C19—C20—C29 | 112.3 (5) |
C3—C4—C23 | 111.3 (4) | C30—C20—C29 | 107.1 (5) |
C24—C4—C5 | 109.3 (4) | C19—C20—C21 | 107.0 (5) |
C3—C4—C5 | 106.9 (4) | C30—C20—C21 | 110.0 (5) |
C23—C4—C5 | 114.9 (4) | C29—C20—C21 | 111.7 (5) |
C6—C5—C4 | 115.8 (4) | C22—C21—C20 | 112.1 (4) |
C6—C5—C10 | 110.9 (4) | C22—C21—H21A | 109.2 |
C4—C5—C10 | 116.9 (3) | C20—C21—H21A | 109.2 |
C6—C5—H5 | 103.7 | C22—C21—H21B | 109.2 |
C4—C5—H5 | 103.7 | C20—C21—H21B | 109.2 |
C10—C5—H5 | 103.7 | H21A—C21—H21B | 107.9 |
C5—C6—C7 | 110.3 (4) | C21—C22—C17 | 114.8 (4) |
C5—C6—H6A | 109.6 | C21—C22—H22A | 108.6 |
C7—C6—H6A | 109.6 | C17—C22—H22A | 108.6 |
C5—C6—H6B | 109.6 | C21—C22—H22B | 108.6 |
C7—C6—H6B | 109.6 | C17—C22—H22B | 108.6 |
H6A—C6—H6B | 108.1 | H22A—C22—H22B | 107.5 |
C8—C7—C6 | 111.6 (3) | C4—C23—H23A | 109.5 |
C8—C7—H7A | 109.3 | C4—C23—H23B | 109.5 |
C6—C7—H7A | 109.3 | H23A—C23—H23B | 109.5 |
C8—C7—H7B | 109.3 | C4—C23—H23C | 109.5 |
C6—C7—H7B | 109.3 | H23A—C23—H23C | 109.5 |
H7A—C7—H7B | 108.0 | H23B—C23—H23C | 109.5 |
C7—C8—C26 | 109.6 (4) | C4—C24—H24A | 109.5 |
C7—C8—C9 | 109.8 (3) | C4—C24—H24B | 109.5 |
C26—C8—C9 | 111.5 (3) | H24A—C24—H24B | 109.5 |
C7—C8—C14 | 109.4 (3) | C4—C24—H24C | 109.5 |
C26—C8—C14 | 107.9 (3) | H24A—C24—H24C | 109.5 |
C9—C8—C14 | 108.7 (3) | H24B—C24—H24C | 109.5 |
C11—C9—C8 | 110.0 (4) | C10—C25—H25A | 109.5 |
C11—C9—C10 | 112.9 (3) | C10—C25—H25B | 109.5 |
C8—C9—C10 | 116.3 (3) | H25A—C25—H25B | 109.5 |
C11—C9—H9 | 105.5 | C10—C25—H25C | 109.5 |
C8—C9—H9 | 105.5 | H25A—C25—H25C | 109.5 |
C10—C9—H9 | 105.5 | H25B—C25—H25C | 109.5 |
C25—C10—C1 | 109.5 (4) | C8—C26—H26A | 109.5 |
C25—C10—C5 | 112.3 (4) | C8—C26—H26B | 109.5 |
C1—C10—C5 | 106.1 (4) | H26A—C26—H26B | 109.5 |
C25—C10—C9 | 113.4 (3) | C8—C26—H26C | 109.5 |
C1—C10—C9 | 106.5 (3) | H26A—C26—H26C | 109.5 |
C5—C10—C9 | 108.6 (3) | H26B—C26—H26C | 109.5 |
C12—C11—C9 | 113.2 (4) | O3—C27—O2 | 121.4 (4) |
C12—C11—H11A | 108.9 | O3—C27—C14 | 124.6 (4) |
C9—C11—H11A | 108.9 | O2—C27—C14 | 114.0 (4) |
C12—C11—H11B | 108.9 | C17—C28—H28A | 109.5 |
C9—C11—H11B | 108.9 | C17—C28—H28B | 109.5 |
H11A—C11—H11B | 107.8 | H28A—C28—H28B | 109.5 |
C13—C12—C11 | 126.7 (4) | C17—C28—H28C | 109.5 |
C13—C12—H12 | 116.6 | H28A—C28—H28C | 109.5 |
C11—C12—H12 | 116.6 | H28B—C28—H28C | 109.5 |
C12—C13—C18 | 119.9 (4) | C20—C29—H29A | 109.5 |
C12—C13—C14 | 119.9 (4) | C20—C29—H29B | 109.5 |
C18—C13—C14 | 120.2 (4) | H29A—C29—H29B | 109.5 |
C13—C14—C27 | 105.2 (4) | C20—C29—H29C | 109.5 |
C13—C14—C15 | 109.7 (4) | H29A—C29—H29C | 109.5 |
C27—C14—C15 | 108.4 (3) | H29B—C29—H29C | 109.5 |
C13—C14—C8 | 111.1 (3) | C20—C30—H30A | 109.5 |
C27—C14—C8 | 110.7 (3) | C20—C30—H30B | 109.5 |
C15—C14—C8 | 111.4 (4) | H30A—C30—H30B | 109.5 |
C16—C15—C14 | 115.1 (4) | C20—C30—H30C | 109.5 |
C16—C15—H15A | 108.5 | H30A—C30—H30C | 109.5 |
C14—C15—H15A | 108.5 | H30B—C30—H30C | 109.5 |
C16—C15—H15B | 108.5 | C31—O4—H4O | 109.5 |
C14—C15—H15B | 108.5 | O4—C31—H31A | 109.5 |
H15A—C15—H15B | 107.5 | O4—C31—H31B | 109.5 |
C15—C16—C17 | 112.1 (4) | H31A—C31—H31B | 109.5 |
C15—C16—H16A | 109.2 | O4—C31—H31C | 109.5 |
C17—C16—H16A | 109.2 | H31A—C31—H31C | 109.5 |
C15—C16—H16B | 109.2 | H31B—C31—H31C | 109.5 |
C10—C1—C2—C3 | −57.4 (5) | C12—C13—C14—C15 | −145.0 (4) |
C1—C2—C3—O1 | −178.2 (4) | C18—C13—C14—C15 | 36.6 (6) |
C1—C2—C3—C4 | 56.6 (6) | C12—C13—C14—C8 | −21.3 (6) |
O1—C3—C4—C24 | 67.2 (5) | C18—C13—C14—C8 | 160.3 (3) |
C2—C3—C4—C24 | −170.2 (5) | C7—C8—C14—C13 | 171.4 (4) |
O1—C3—C4—C23 | −49.5 (5) | C26—C8—C14—C13 | −69.4 (5) |
C2—C3—C4—C23 | 73.0 (5) | C9—C8—C14—C13 | 51.6 (4) |
O1—C3—C4—C5 | −175.7 (3) | C7—C8—C14—C27 | 54.9 (4) |
C2—C3—C4—C5 | −53.1 (5) | C26—C8—C14—C27 | 174.1 (3) |
C24—C4—C5—C6 | −56.2 (5) | C9—C8—C14—C27 | −64.9 (4) |
C3—C4—C5—C6 | −171.9 (4) | C7—C8—C14—C15 | −65.9 (4) |
C23—C4—C5—C6 | 64.1 (5) | C26—C8—C14—C15 | 53.3 (4) |
C24—C4—C5—C10 | 170.2 (4) | C9—C8—C14—C15 | 174.3 (3) |
C3—C4—C5—C10 | 54.5 (5) | C13—C14—C15—C16 | −40.9 (5) |
C23—C4—C5—C10 | −69.5 (5) | C27—C14—C15—C16 | 73.5 (5) |
C4—C5—C6—C7 | 160.7 (4) | C8—C14—C15—C16 | −164.4 (4) |
C10—C5—C6—C7 | −63.0 (5) | C14—C15—C16—C17 | 56.1 (6) |
C5—C6—C7—C8 | 62.1 (5) | C15—C16—C17—C28 | 60.5 (5) |
C6—C7—C8—C26 | 70.0 (5) | C15—C16—C17—C22 | 178.7 (4) |
C6—C7—C8—C9 | −52.7 (5) | C15—C16—C17—C18 | −60.3 (5) |
C6—C7—C8—C14 | −171.8 (3) | C12—C13—C18—C17 | 136.2 (4) |
C7—C8—C9—C11 | 177.9 (4) | C14—C13—C18—C17 | −45.4 (5) |
C26—C8—C9—C11 | 56.3 (5) | C12—C13—C18—C19 | −95.1 (5) |
C14—C8—C9—C11 | −62.5 (4) | C14—C13—C18—C19 | 83.3 (5) |
C7—C8—C9—C10 | 47.9 (4) | C28—C17—C18—C13 | −68.2 (5) |
C26—C8—C9—C10 | −73.7 (4) | C16—C17—C18—C13 | 54.0 (5) |
C14—C8—C9—C10 | 167.5 (3) | C22—C17—C18—C13 | 174.9 (4) |
C2—C1—C10—C25 | −67.3 (5) | C28—C17—C18—C19 | 163.7 (4) |
C2—C1—C10—C5 | 54.2 (5) | C16—C17—C18—C19 | −74.1 (5) |
C2—C1—C10—C9 | 169.8 (3) | C22—C17—C18—C19 | 46.8 (5) |
C6—C5—C10—C25 | −71.5 (5) | C13—C18—C19—C20 | 177.5 (4) |
C4—C5—C10—C25 | 64.2 (5) | C17—C18—C19—C20 | −53.8 (6) |
C6—C5—C10—C1 | 168.9 (3) | C18—C19—C20—C30 | 175.2 (5) |
C4—C5—C10—C1 | −55.4 (5) | C18—C19—C20—C29 | −66.5 (6) |
C6—C5—C10—C9 | 54.7 (5) | C18—C19—C20—C21 | 56.4 (6) |
C4—C5—C10—C9 | −169.5 (3) | C19—C20—C21—C22 | −56.3 (6) |
C11—C9—C10—C25 | −51.6 (5) | C30—C20—C21—C22 | −174.3 (5) |
C8—C9—C10—C25 | 77.0 (4) | C29—C20—C21—C22 | 67.0 (6) |
C11—C9—C10—C1 | 68.9 (4) | C20—C21—C22—C17 | 55.6 (7) |
C8—C9—C10—C1 | −162.5 (3) | C28—C17—C22—C21 | −168.2 (5) |
C11—C9—C10—C5 | −177.2 (4) | C16—C17—C22—C21 | 70.1 (6) |
C8—C9—C10—C5 | −48.6 (5) | C18—C17—C22—C21 | −49.1 (6) |
C8—C9—C11—C12 | 41.8 (5) | C13—C14—C27—O3 | −42.0 (6) |
C10—C9—C11—C12 | 173.6 (4) | C15—C14—C27—O3 | −159.3 (4) |
C9—C11—C12—C13 | −10.6 (7) | C8—C14—C27—O3 | 78.1 (5) |
C11—C12—C13—C18 | 178.7 (4) | C13—C14—C27—O2 | 139.9 (4) |
C11—C12—C13—C14 | 0.3 (7) | C15—C14—C27—O2 | 22.5 (5) |
C12—C13—C14—C27 | 98.5 (5) | C8—C14—C27—O2 | −100.0 (4) |
C18—C13—C14—C27 | −79.8 (5) |
D—H···A | D—H | H···A | D···A | D—H···A |
O2—H2···O4 | 0.82 | 1.82 | 2.619 (5) | 164 |
O1—H1···O3i | 0.82 | 2.17 | 2.991 (5) | 178 |
O4—H4O···O1i | 0.82 | 1.96 | 2.755 (5) | 164 |
C9—H9···O3 | 0.98 | 2.36 | 3.122 (5) | 134 |
C15—H15B···O2 | 0.97 | 2.27 | 2.649 (5) | 102 |
C24—H24A···O1 | 0.96 | 2.58 | 2.980 (6) | 105 |
Symmetry code: (i) x−1/2, −y+1/2, −z+1. |
Experimental details
Crystal data | |
Chemical formula | C30H48O3·CH4O |
Mr | 488.73 |
Crystal system, space group | Orthorhombic, P212121 |
Temperature (K) | 296 |
a, b, c (Å) | 7.538 (2), 13.710 (3), 27.629 (8) |
V (Å3) | 2855.3 (13) |
Z | 4 |
Radiation type | Mo Kα |
µ (mm−1) | 0.07 |
Crystal size (mm) | 0.58 × 0.40 × 0.24 |
Data collection | |
Diffractometer | Siemens P4 diffractometer |
Absorption correction | – |
No. of measured, independent and observed [I > 2σ(I)] reflections | 3492, 3203, 1876 |
Rint | 0.021 |
(sin θ/λ)max (Å−1) | 0.617 |
Refinement | |
R[F2 > 2σ(F2)], wR(F2), S | 0.057, 0.158, 0.89 |
No. of reflections | 3203 |
No. of parameters | 316 |
H-atom treatment | H-atom parameters constrained |
Δρmax, Δρmin (e Å−3) | 0.21, −0.19 |
Computer programs: XSCANS (Siemens, 1994), XSCANS, SHELXS97 (Sheldrick, 1997), SHELXL97 (Sheldrick, 1997), SHELXTL/PC (Siemens, 1991), SHELXL97.
O1—C3 | 1.449 (5) | C12—C13 | 1.336 (6) |
O2—C27 | 1.323 (5) | O4—C31 | 1.369 (8) |
O3—C27 | 1.213 (5) | ||
O1—C3—C2 | 107.5 (4) | C12—C13—C14 | 119.9 (4) |
O1—C3—C4 | 112.4 (4) | C18—C13—C14 | 120.2 (4) |
C6—C5—C4 | 115.8 (4) | C13—C14—C27 | 105.2 (4) |
C4—C5—C10 | 116.9 (3) | C16—C15—C14 | 115.1 (4) |
C8—C9—C10 | 116.3 (3) | O3—C27—O2 | 121.4 (4) |
C13—C12—C11 | 126.7 (4) | O3—C27—C14 | 124.6 (4) |
C12—C13—C18 | 119.9 (4) | O2—C27—C14 | 114.0 (4) |
C1—C2—C3—O1 | −178.2 (4) | C15—C14—C27—O3 | −159.3 (4) |
O1—C3—C4—C24 | 67.2 (5) | C8—C14—C27—O3 | 78.1 (5) |
O1—C3—C4—C23 | −49.5 (5) | C13—C14—C27—O2 | 139.9 (4) |
O1—C3—C4—C5 | −175.7 (3) | C15—C14—C27—O2 | 22.5 (5) |
C13—C14—C27—O3 | −42.0 (6) | C8—C14—C27—O2 | −100.0 (4) |
D—H···A | D—H | H···A | D···A | D—H···A |
O2—H2···O4 | 0.82 | 1.82 | 2.619 (5) | 164 |
O1—H1···O3i | 0.82 | 2.17 | 2.991 (5) | 178 |
O4—H4O···O1i | 0.82 | 1.96 | 2.755 (5) | 164 |
C9—H9···O3 | 0.98 | 2.36 | 3.122 (5) | 134 |
C15—H15B···O2 | 0.97 | 2.27 | 2.649 (5) | 102 |
C24—H24A···O1 | 0.96 | 2.58 | 2.980 (6) | 105 |
Symmetry code: (i) x−1/2, −y+1/2, −z+1. |
Astilbe chinensis (Haxim.) Franch. et Savat. (Saxiffagaceae) is a perennial herbaceous plant growing at an altitude of 390–3600 m in China, Russia, Japan and Korea. Its rhizome, known as 'Luo Xinfu' (Chinese name), has been used for headache, arthralgia, chronic bronchitis and stomachalgia, in traditional Chinese medicine (Pan, 1985, 1995). Pharmacological experiments have indicated that the extracts from A. chinensis had antineoplastic and immunopotent activities (Chen et al., 1996). Previously, we isolated β-sitosterol palmitate, daucosterol, β-sitosterol and bergenin from the rhizome of A. chinensis (Sun et al., 2002). To investigate the bioactive natural products from A. chinensis, chemical studies on the rhizome of A. chinensis were undertaken by screening using antineoplastic tests in vitro and we have obtained cytotoxic and apoptotic-inducing triterpenoid 3β-hydroxyolean-12-en-27-oic acid, (I), from its petroleum ether extract. It exhibits significant cytotoxic activity against human ovarian carcinoma cell line (HO-8910), human cervical squamous carcinoma cell line (HeLa) and human leukemia cell (HL60) in vitro, its 50% inhibitory concentration (IC50) values were 8.0 (9), 3.94 (13) and 3.67 (19) µg ml−1, respectively. Moreover, it induces apoptotic cell death of the HO-8910 in a dose-dependent manner, ranging from 2.5 µg mL−1 to 40.0 µg mL−1. The structure of the title compound, (I), was elucidated by spectroscopic analysis including two-dimensional NMR spectroscopy and confirmed by single-crystal X-diffraction analysis.
The structure of (I) with the atom-numbering scheme is shown in Fig. 1. The molecule is composed of five six-membered rings, viz. A (C1—C5/C10), B (C5—C10), C (C8/C9/C11—C14), D (C13—C18) and E (C17—C22). Rings A, B, D and E adopt chair conformations whereas ring C adopts a slightly distorted half-chair conformation owing to the C12═C13 double bond. All rings are trans fused except for D/E junction, which is cis fused. The orientation of the carboxy group is axial and that for hydroxy group is equatorial.
The hydroxy and carboxy groups both serve as simultaneous hydrogen-bond donors and acceptors (Table 2). In the asymmetric unit, the two molecules are linked by O2—H2···O4 hydrogen bonds. In the solid state, the screw-related molecules are linked by O1—H1···O3i hydrogen bonds (Table 2), to form molecular chains along the a axis. The chain formation is further stabilized by the methanol solvate through O4—H4O···O1i hydrogen bonds. The crystal structure is further stabilsed by C—H···O interactions.