 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](en5079fig1mag.jpg)
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Figure 1
(a) Gel-filtration chromatogram of mCAR LBD–SRC1 complex and mCAR LBD. (b) SDS–PAGE, 15% gel. Lane 1, molecular-weight markers. Lane 2, soluble extract from E. coli. Lane 3, pelleted fraction from E. coli. Lane 4, Ni–NTA flowthrough upon loading of mCAR LBD and mCAR LBD–SRC1. Lane 5, Ni–NTA flowthrough post-wash (20 mM Tris, 150 mM NaCl, 2.5 mM CaCl2 pH 8.4 at room temperature) of mCAR LBD–SRC and mCAR LBD-bound Ni–NTA resin. Lane 6, Ni–NTA column post-protein elution. Lane 7, fraction 42 of gel-filtration chromatogram containing mCAR LBD–SRC1 complex. Lane 8, fraction 58 of gel-filtration chromatogram containing purified mCAR LBD. |
![[Figure 1]](en5079fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
