Figure 1 The protein and DNA constructs used in characterization and crystallization. (a) Human HNF4-DBD protein sequence. The predicted DNA-recognition helix is boxed and the MODY mutations are shown in red. Cloning artifacts from the expression vector are indicated by lower case letters at the N-terminal end. (b) The natural HNF1 promoter sequence (-46 to -66; Gragnoli et al., 1997) containing the HNF4 recognition site used in crystallization and binding studies. Two direct-repeat half-sites are boxed and the MODY mutation is shown in red.

© International Union of Crystallography 2008