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Figure 4 EMSA of NMB1585-DNA complexes. Increasing amounts of purified NMB1585 protein (20-640 nM) were incubated with a 378 bp DNA probe (20 nM) PCR-amplified from the promoter region of the NMB1585 gene in either the absence (lanes 2-8) or presence (lanes 9-14) of 10 mM sodium salicylate. The DNA-protein complexes were analysed by gel electrophoresis on a tris-borate-EDTA polyacrylamide gel and stained for DNA using SYBR green. Samples are as follows: DNA only (lanes 1 and 8), 20 nM protein (lanes 2 and 9), 40 nM protein (lanes 3 and 10), 80 nM protein (lanes 4 and 11), 160 nM protein (lanes 5 and 12), 320 nM protein (lanes 6 and 13) and 640 nM protein (lanes 7 and 14).