Overexpression, purification and preliminary X-ray diffraction analysis of the controller protein C.Csp231I from Citrobacter sp. RFL231

Streeter, S.D.; McGeehan, J.E.; Kneale, G.G.

doi:10.1107/S1744309109028681

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 3
EMSA of C.Csp231I with hex-labelled 96 bp duplex DNA. Increasing concentrations of C.Csp231I were incubated with a hex-labelled 96 bp duplex DNA fragment located upstream of the csp231IC start codon at protein:DNA ratios of 0:1, 1:1, 2:1 and 4:1. The DNA concentration was 800 nM throughout.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 65| Part 9| August 2009| Pages 898-901

doi:10.1107/S1744309109028681

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