Expression, purification, crystallization and preliminary X-ray analysis of wild-type and of an active-site mutant of glyceraldehyde-3-phosphate dehydrogenase from Campylobacter jejuni

Tourigny, D.S.; Elliott, P.R.; Edgell, L.J.; Hudson, G.M.; Moody, P.C.E.

doi:10.1107/S1744309110044465

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Crystals of wild-type cGAPDH grown in 1 mM NADP⁺ using the Omza 1K and 4K Salt Screens (Emerald BioSystems). Crystals grew in 500 nl sitting drops with 20%(w/v) PEG 4K (a–c) or PEG 1K (d) as precipitant and various salts added to a final concentration of 200 mM: ammonium fluoride (a), magnesium acetate (b), sodium tartrate (c) and sodium/potassium tartrate (d). Crystals from (d) were used in data collection.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 67| Part 1| December 2011| Pages 72-75

doi:10.1107/S1744309110044465

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