 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](sw5049fig1mag.jpg)
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Figure 1
DSF melting curves, their first derivatives and representative crystallization images for the various stages of purification optimization. Transitions at 318, 341 and 359 K correspond to misfolded, apo and PLP-loaded BioA, respectively. (a) BioA as purified according to Dey et al. (2010  ) with PLP and Tris in the lysis buffer and Tris and DTT in the storage buffer. (b) BioA purified with PLP and HEPES in the lysis buffer and Tris and DTT in the storage buffer. (c) BioA purified with PLP and HEPES in the lysis buffer and HEPES and TCEP in the storage buffer. (d) BioA purified as in (c) but with PLP supplementation prior to final concentration (Shi et al., 2011). |
![[Figure 1]](sw5049fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
