Biophysical characterization of TraMΔ. (a) TraMΔ elutes as a single peak from the Superdex 200 size-exclusion column. The 280 nm (solid line) and 254 nm (dotted line) readings are shown. A standard (BioRad) is shown with its molecular weight (discontinued). (b) In the monodispersity analysis via DLS TraMΔ appears as a narrow peak.