Crystallization and preliminary structure determination of the transfer protein TraM from the Gram-positive conjugative plasmid pIP501

Goessweiner-Mohr, N.; Grumet, L.; Pavkov-Keller, T.; Birner-Gruenberger, R.; Grohmann, E.; Keller, W.

doi:10.1107/S1744309113000134

Figure 4
CD analysis of TraM [Delta]

. (a) TraM [Delta]

is folded in solution. The black curve represents the average of ten individual wavelength scans. The standard deviation is displayed as a shaded area. (b) Secondary structure content of TraM [Delta]

. The NRMSD (normalized root mean square deviation) is 0.018. (c) TraM [Delta]

unfolding and refolding characteristics. The CD spectra are shown at 298 and 368 K and after cooling to 298 K (left panel). The temperature scan at 208 nm (up- and down-scan) is shown in the right panel.