Images of 30S microcrystals and preliminary SFX diffraction image of 30S ribosomal subunits. (a) Long needles (3 × 5 × 200 µm) of 30S microcrystals before pre-filtration. (b) 30S microcrystals broken after filtration. (c) SFX diffraction image collected on a CSPAD detector extending to beyond 6 Å resolution, with unit-cell parameters a = b = 405, c = 177 Å, α = β = γ = 90°. (d) The same image as in (c) with the reflection predictions after indexing.