DNA groove-width analysis of the 25OL DNA. Groove-width analysis of the 25OL DNA (cyan) compared with the published 35OL+R complex (PDB entry 3clc; magenta; McGeehan et al., 2008). Upper curve, major groove; lower curve, minor groove. The DNA sequence of the 25OL sequence is shown below. The TATA sequences are shown in bold and the DNA recognition bases are underlined.