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Figure 5
The hanging-drop vapor-diffusion method is illustrated schematically. The components of the drop and reservoir, and the physical equilibration process, are the same here as for the sitting drop. The exception is that the protein drop is suspended from a cover slip over the reservoir rather than resting on a surface. Plasticware for carrying out both sitting- and hanging-drop vapor diffusion are widely and commercially available in numerous formats.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 70| Part 1| December 2014| Pages 2-20
doi:10.1107/S2053230X13033141
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