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Figure 2
(a) Size-exclusion chromatography trace (HiLoad 16/600 Superdex 200 75 pg; GE Healthcare). CagXct elutes at 80–110 ml, which is in agreement with its monomer molecular mass of 11.4 kDa. The peak intensity is 192 mAU. Samples between 80 and 110 ml labelled 3–17 in red were picked for 15% SDS–PAGE analysis. (b) 15% SDS–PAGE analysis of the purified CagXct samples labelled 3, 5, 9, 11, 13, 15 and 17 in (a). Lane M contains molecular-mass marker (labelled in kDa).

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 73| Part 3| March 2017| Pages 167-173
https://doi.org/10.1107/S2053230X17001376
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