 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](us5124fig2mag.jpg)
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Figure 2
(a) 15% SDS–PAGE analysis of PlaA. Lane M, low-molecular-weight markers; lanes 1–13, purified recombinant PlaA. (b) Analytical gel-filtration chromatography. Separation of PlaA (red curve) and PlaA with PC (blue curve) was carried out using a Superdex 75 HR 10/300 GL column in 20 mM Tris–HCl pH 8.0, 200 mM NaCl. The curve for protein markers is shown in black. (c) PlaA was incubated with lipids to determine enzymatic activities via quantification of the release of fatty acid (FFA), and the lipids were then extracted and analyzed using TLC. All results shown are representative of at least one additional experiment. st, standard FFA. (d) ITC measurements of PlaA with PC. Heat release upon protein injection (top), integrated heat plotted against the molar ratio of ligand and protein (bottom) and the resulting disassociation constant (Kd) are shown. All experiments were performed at least three times independently. |
![[Figure 2]](us5124fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
