Figure 1
Schematic drawing of the modified vapor-controlling-diffusion method. The drawing represents only one well of a Linbro plate and shows its assembly for the respective experiment. The individual set-up is sealed with petroleum jelly (or high-vacuum grease) and a coverslip. (a) Conventional vapor-diffusion hanging-drop crystallization. (b) Modified vapor-diffusion hanging-drop crystallization with a funnellet barrier (left) or a PARA-FILM hole barrier (right). (c) Modified vapor-diffusion sitting-drop crystallization. |