Surface treatment by oxidizing the plates can alter the response of protein crystallization

Guo, Y.-Z.; Liu, Y.-M.; Yin, D.-C.; He, J.; Zhang, C.-Y.; Cao, H.-L.; Hou, H.; Ma, W.; Guo, W.-H.; Shi, J.-Y.; Cui, C.; Wang, Z.; Shang, P.

doi:10.1107/S1600576713030999

Journal of Applied Crystallography Journal of Applied
Crystallography

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Figure 3
Typical morphology of the crystals grown in the OCPs and control plates. (a)–(d) Images of crystallization droplets in the OCP group. (a′)–(d′) Images of crystallization droplets in the control group. (a), (a′) Catalase: 10 mg ml⁻¹; crystallization conditions: F6. (b), (b′) α-Chymotrypsinogen: 10 mg ml⁻¹; crystallization conditions: D4. (c), (c′) Concanavalin A: 10 mg ml⁻¹; crystallization conditions: D4. (d), (d′) Glucose isomerase: 3.5 mg ml⁻¹; crystallization conditions: F4. The Index crystallization screening kit from Hampton Research Company was used. The crystals grown in the OCPs usually exhibited better morphology (larger size and a higher number of well defined faces) than the crystals grown in the control plates.

JOURNAL OF
APPLIED
CRYSTALLOGRAPHY

ISSN: 1600-5767

Volume 47| Part 1| February 2014| Pages 228-236

doi:10.1107/S1600576713030999

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