issue contents

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047

August 1999 issue

Highlighted illustration

Cover illustration: A ribbon diagram of the polypeptide backbone for the entire tetramer (Mr = 260 000) of beef liver catalase containing four heme groups and four NADPH coenzyme molecules (p. 1383).

research papers


link to html
The crystal structures of the peanut-lectin complexes of methyl-β-galactose and N-acetyllactosamine and those involving other sugars demonstrate the important role of water molecules in generating carbohydrate specificity. A comparative study also provides a structural explanation for the exclusive specificity of peanut lectin for galactose at the monosaccharide level as against that of other related lectins for galactose and N-acetylgalactosamine.

link to html
The structure of orthorhombic beef liver catalase crystals was determined by X-ray crystallography and studied as well by atomic force microscopy. Combination of the two techniques provided explanations of numerous features of the crystal growth process.


link to html
The crystal structures of three double-stranded RNA heptamers derived from the tRNAAla acceptor stem were refined as pseudo-merohedral twins at high resolution.

link to html
The frequency of occurrence of X in the consensus glycosylating sequence Asn-X-Ser/Thr in proteins and the conformational and hydrogen-bonding features of the triplet have been analysed using the PDB database.

link to html
The geometry of interaction of different aromatic side chains with the indole ring of tryptophan is presented.

link to html
A method for the simplification of the solution of MIR derivative sites and the improvement of isomorphism using a derivative backsoak is described. MIR sites are depleted, soak/backsoak difference Pattersons are simpler and the subset of sites may be used as a starting point for cross-phasing.

link to html
Geometrical data for Ca, Mg, Mn, Fe, Cu and Zn bonded to ligands which are analogues of amino-acid side chains have been extracted from the Cambridge Structural Database and tabulated. These could be useful in the determination, refinement or assessment of metalloprotein structures, or in the understanding of function.

link to html
Precipitants can be classified into three types for one particular protein. Combination of hard and soft precipitants has many advantages in protein crystallization.

link to html
The feasibility of two-wavelength MAD experiments and the optimal wavelength choice are discussed.

crystallization papers


link to html
Three different crystal forms of the minor group human rhinovirus serotype 2 (HRV2) hase been obtained. Crystals belonging to space group I222 diffracted beyond 1.8 Å resolution and contained 15 protomers in the asymmetric unit. Structure refinement on this crystal form is now in progress.

link to html
Purple acid phosphate from pig allantoic fluid has been crystallized in the space group P212121 with unit-cell dimensions a = 66.8, b = 70.3 and c = 77.1 Å. Using cryocooling techniques a complete data set to 1.55 Å resolution has been obtained.

link to html
Crystals of ROO, a terminal oxidase capable of reducing dioxygen to water, have been characterized. The non-crystallographic twofold axis within the homodimer is parallel to a twinning axis found in pseudo-merohedrally twinned crystals of ROO.

link to html
Crystals of potent inhibitor of blood platelet activation have been grown. Molecular replacement has not been successful in producing a trial structure, most likely because of poor diffraction.


link to html
The substrate-free E. coli tyrosine amintotransferase (eTAT) bound with a cofactor pyridoxal 5′-phosphate (PLP) was crystallized in trigonal space group P32. The low-resolution crystal structure shows that the substrate-free eTAT has a tertiary structure similar to the open form of aspartate aminotransferase.

link to html
Sterol carrier protein 2 was expressed in Escherichia coli and purified to electrophoretic homogeneity. Single crystals were obtained suitable for high-resolution X-ray structure analysis.

link to html
An essential protein in bacterial cell-wall biosynthesis and vancomycin resistance in the clinically important bacterium E. faceium BM4147 has been expressed and crystallized in a form suitable for X-ray structure determination.

link to html
Crystals of rat m-calpain have been obtained in two forms. Diffraction data have been collected to 2.6 Å for the P1 form and to 2.15 Å for the P21 form.

link to html
Crystallization and crystallographic study of HIP/PAP, a human C-lectin overexpressed in primary liver cancer. Presentation of a molecular-replacement solution using the human lithostathine structure and the AMoRe software.
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds