Platinum-induced space-group transformation in crystals of the platelet glycoprotein Ibα N-terminal domain

The interaction between platelet glycoprotein (GP) Ibα and von Willebrand factor is essential for thrombus formation, leading to the arrest of bleeding. The crystal structure of the N-terminal domain of GP Ibα has been solved at 1.7 Å resolution.

The role of solvent transport in cryo-annealing of macromolecular crystals

Annealing experiments with flash-cooled protein crystals suggest that water is transported into or out of the crystal during the melting phase, depending on the ambient humidity. Measurements of the bulk density of cryosolvents suggest that during successful annealing the water transport modulates the thermal contraction of the cryosolvent in order to compensate for the thermal contraction of the protein crystal lattice.

Structures of d(GCGAAGC) and d(GCGAAAGC) (tetragonal form): a switching of partners of the sheared G·A pairs to form a functional G·A×A·G crossing

The DNA fragments d(GCGAAGC) form a new type of bulge-in duplex with the G·A×A·G crossing in the central part, to which an addition of an A residue induces exchange of the partners of G·A pairs to form a base-intercalated duplex.

Likelihood-enhanced fast rotation functions

Likelihood-enhanced fast rotation functions have been developed and shown to be more sensitive to the correct orientation than the traditional fast rotation functions, especially when information from a partial solution can be exploited.

Structural insight into nucleotide recognition in tau-protein kinase I/glycogen synthase kinase 3β

The first two crystal structures of binary complexes of full-length glycogen synthase kinase 3β with the ATP analogues hydrolysate ADP and AMPPNP are described. Although several other crystal structures of the enzyme have already been reported, none of them contain ATP analogues. The structures of these binary complexes reveal detailed features of nucleotide recognition by GSK3β and may have useful implications in the design of drug candidates against Alzheimer's disease.

Structure determination of a truncated dimeric splicing endonuclease in pseudo-face-centered space group P2₁2₁2

The molecular replacement solution of a dimeric splicing endonuclease in pseudo-face-centered space group P2₁2₁2 is reported.

Complexed and ligand-free high-resolution structures of urate oxidase (Uox) from Aspergillus flavus: a reassignment of the active-site binding mode

High-resolution structures of urate oxidase–inhibitor complexes lead to a reformulation of the active-site topology.

Structural study of the type II 3-de­hydroquinate dehydratase from Actinobacillus pleuropneumoniae

The structure of the type II dehydroquinate dehydratase (DHQase) from A. pleuropneumoniae, the third enzyme of the shikimate pathway, has been determined.

Crystal packing in two pH-dependent crystal forms of rhamnogalacturonan acetylesterase

The glycoprotein rhamnogalacturonan acetylesterase has been crystallized in two different polymorphic modifications: an orthorhombic crystal form which appeared at pH 5.0 and a trigonal form which was formed at pH 4.5. The differences in crystal packing can be explained by the pH-dependent variation in the protonation state of glutamic acids on the protein surface.

Enzymatic and structural characterization of non-peptide ligand–cyclophilin complexes

Complexes of two piperidine ligands provide the first non-peptide cyclophilin–inhibitor structures. Enzymatic solution studies show K_i values of greater than 20 mM.

Structure of the biotin carboxylase subunit of pyruvate carboxylase from Aquifex aeolicus at 2.2 Å resolution

The crystal structure of the biotin carboxylase subunit of pyruvate carboxylase from A. aeolicus (PC-β) was determined at 2.2 Å resolution. One PC-β molecule is contained in the asymmetric unit with space group P2₁2₁2; electron density for domain B was clearly shown in the final model.

The structure and refinement of apocrustacyanin C₂ to 1.3 Å resolution and the search for differences between this protein and the homologous apoproteins A₁ and C₁

The lobster crustacyanin C₂ protein subunit structure is reported at 1.3 Å resolution. A search for chemical structure post-translational modifications was made using three B-factor plot evaluations, but none proved sensitive enough to explain the C₂ protein chromatography signature differences from the homologous A₁ or C₁ protein subunits.

Monitoring the anomalous scattering signal and noise levels in X-ray diffraction of crystals

An index R_as is proposed based on analysis of the Bijvoet differences of acentric and centric reflections to better estimate the accuracy and strength of the anomalous scattering signal in data.

Structure of Escherichia coli YfdW, a type III CoA transferase

Crystal structures are reported for free and coenzyme A (CoA) bound forms of the YfdW protein from E. coli, a representative type III CoA transferase.

Preparation and X-ray crystallographic analysis of the Ca²⁺-discharged photoprotein obelin

Crystals of the Ca²⁺-discharged W92F mutant of obelin from Obelia longissima have been grown, representing the first crystallization of a photoprotein after the Ca²⁺-triggered bioluminescence and bound with product, coelenteramide.

Crystallization and preliminary X-ray crystallographic studies of the small form of glucose-inhibited division protein A from Thermus thermophilus HB8

The small form of glucose-inhibited division protein A from T. thermophilus HB8 has been crystallized and its diffraction data have been collected to 1.65 Å resolution. Crystals belong to the trigonal space group P3₁21 or P3₂21.

Crystallization of Pseudomonas aeruginosa AHL synthase LasI using β-turn crystal engineering

The acyl-homoserinelactone synthase LasI has been crystallized in space group F23, with unit-cell parameters a = b = c = 154.90 Å. Crystals were obtained using a β-turn engineering approach.

Expression and crystallization of several forms of the Propionibacterium shermanii transcarboxylase 5S subunit

The crystallizations of three different forms of the dimeric outer 5S subunit of transcarboxylase differ in their requirement for the addition of lithium sulfate.

Cloning, purification, crystallization and X-ray analysis of the Escherichia coli pyrimidine nucleoside hydrolase YeiK

The E. coli yeiK gene product has been overexpressed in recombinant form and characterized as a pyrimidine nucleoside hydrolase. Crystals diffracting to 1.7 Å resolution are reported.

Purification, crystallization and preliminary X-ray analysis of native and selenome­thionine class I tagatose-1,6-bis­phosphate aldolase from Streptococcus pyogenes

Recombinant class I D-tagatose-1,6-bisphosphate aldolase from S. pyogenes was expressed in E. coli, purified and crystallized. Crystals of both native and selenomethionine isoforms were obtained and are suitable for structural analysis.

Crystallization and preliminary X-ray analysis of Aes, an acetyl-esterase from Escherichia coli

Aes has been crystallized as an N-­terminally His₆-tagged protein both in the native form and with selenomethionine substitution.

Expression, purification and preliminary crystallographic analysis of phosphoribosyl isomerase (PriA) from Streptomyces coelicolor

A novel bifunctional enzyme, phosphoribosyl isomerase, involved in both the histidine- and tryptophan-biosynthesis pathways, has been crystallized. Crystals diffract to 2 Å resolution and are suitable for X-ray structure determination.

Crystallization and preliminary X-ray analysis of GlcNAcα1,4Gal-releasing endo-β-galactosidase from Clostridium perfringens

The unique clostridial endo-β-galactosidase (Endo-β-GalGnGa) capable of releasing the disaccharide GlcNAcβ1,4Gal from O-glycans expressed in the gastric gland mucous cell-type mucin has been crystallized.

Crystallization and phasing of focal adhesion protein 52 from Gallus gallus

Focal adhesion protein 52 (FAP52) is a multidomain adaptor protein composed of an N-terminal α-helical domain and C-terminal SH3 domain connected by a predicted unstructured region. FAP52 was crystallized in two crystal forms and initial phase information was obtained from xenon-pressurized crystals using diffraction data collected at two softer X-ray wavelengths.

Crystallization and preliminary X-ray analysis of a novel Trichoderma reesei xylanase IV belonging to glycoside hydrolase family 5

Xylanase IV from T. reesei has been crystallized and X-ray diffraction data have been collected to 2.2 Å resolution.

A new crystal form of XT6 enables a significant improvement of its diffraction quality and resolution

The extracellular xylanase from Geobacillus stearothermophilus T-6 (XT6) has recently been crystallized in a new monoclinic crystal form. The new crystals diffract to better than 1.5 Å resolution and show significantly improved crystallographic properties as compared with the previous crystals of XT6 of the hexagonal form.

Crystallization and preliminary crystallographic studies of the NCoA-1/SRC-1 PAS-B domain bound to the LXXLL motif of the STAT6 transactivation domain

Crystallographic analysis of the first complex between a PAS domain and another protein.

Crystallization and preliminary X-ray diffraction study of fructan 1-exo­hydrolase IIa from Cichorium intybus

Crystals of fructan exohydrolase IIa from C. intybus have been prepared and proved to be suitable for successful structural analysis.

Crystallization and preliminary X-ray diffraction study of two complexes of a TAXI-type xylanase inhibitor with glycoside hydrolase family 11 xylanases from Aspergillus niger and Bacillus subtilis

Crystals of two complexes of an endoxylanase inhibitor from T. aestivum L. with a family 11 xylanase have been prepared. The crystals of the complex TAXI I–A. niger xylanase as well as the crystals of TAXI I in complex with B. subtilis xylanase proved to be suitable for successful structural analysis.

Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a:K30

A novel integral membrane lipoprotein, Wza, from E. coli serotype O9a:K30 has been purified and crystallized.

Crystallization and preliminary crystallographic analysis of the Escherichia coli water channel AqpZ

AqpZ is a 24 kDa integral membrane protein that facilitates water movement across the plasma membrane of E. coli. The first crystallization and preliminary X-ray analysis of AqpZ are described in this study.

Crystallization and preliminary X-ray crystallographic analysis of the C-terminal domain of ParC protein from Bacillus stearothermophilus

The 36 kDa C-terminal domain of ParC from B. stearothermophilus has been crystallized in the trigonal space group P3₁ (or P3₂), with unit-cell parameters a = b = 83.5, c = 45.1 Å. A complete native data set to 2.0 Å has been collected.

Cloning, expression, purification, crystallization and preliminary X-ray analysis of human liver glyceraldehyde-3-phosphate de­hydrogenase

Human liver glyceraldehyde-3-phosphate dehydrogenase was purified and crystallized. Crystals belong to space group P2₁, with unit-cell parameters a = 63.23, b = 97.84, c = 84.23 Å, β = 104.1°, and show diffraction to 2.8 Å resolution.

Crystallization and preliminary X-ray crystallographic analysis of macrophage/microglia-specific calcium-binding protein Iba1

Macrophage/microglia-specific calcium-binding protein Iba1s from human and mouse were overexpressed and crystallized. A preliminary X-ray crystallographic analysis of the crystals is reported.

Crystallization and preliminary X-ray study of isomaltodextranase from Arthrobacter globiformis

Monoclinic isomaltodextranase crystals which diffract X-rays to 1.8 Å resolution on a rotating-anode generator were grown from PEG 6000 and ammonium sulfate.

Expression, purification and preliminary X-ray characterization of histidinol phosphate phosphatase

A recombinant form of histidinol phosphate phosphatase from T. thermophilus HB8 has been expressed in E. coli, purified and crystallized in crystal forms I and II, belonging to space groups P2₁2₁2 and C222₁, respectively. Diffraction data were collected to 1.70 and 1.75 Å resolution for crystal forms I and II, respectively.

Crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3

Inorganic pyrophosphatase from the hyperthermophile P. horikoshii has been crystallized by the hanging-drop vapour-diffusion method at pH 5.0 using polyethyleneglycol 4000 as the precipitant.

Purification, crystallization and preliminary X-ray analysis of the GTP-binding protein Rab9 implicated in endosome-to-TGN vesicle trafficking

Growth of hemihedrally twinned crystals of the small GTP-binding protein Rab9, which is implicated in vesicular transport from endosomes to the trans-Golgi network, in complex with GDP is described.

Crystallization and preliminary X-ray analysis of family 39 β-D-xylosidase from Geobacillus stearothermophilus T-6

The crystallization and preliminary X-ray analysis of the β-D-xylosidase from G. stearothermophilus T-6, a family 39 glycoside hydrolase, is described. Purified native and selenomethionine-containing enzymes were crystallized and belong to space group P2₁2₁2₁. The crystal diffracted X-rays to a resolution of 2.1 Å.

Crystallization and preliminary X-ray study of γ-type cyclodextrin glycosyltransferase from Bacillus clarkii

A γ-type cyclodextrin glycosyltransferase that produces γ-cyclodextrin as the main product has been crystallized and analyzed by X-ray diffraction. Phasing was successfully carried out using the molecular-replacement method.

Production, crystallization and preliminary X-ray crystallographic studies of the bacteriophage φ12 packaging motor

Native and selenomethionyl-labelled protein of the ssRNA-packaging ATPase from bacteriophage φ12 crystallizes in space groups I222 and P1. Data have been collected for both space groups, to 2.5 and 2.0 Å resolution, respectively.

Expression, crystallization and preliminary X-ray analysis of extracellular modules of the neural cell-adhesion molecules NCAM and L1

NCAM Ig1-4, NCAM Ig1-5 and the whole extracellular part of L1 were crystallized. Preliminary data have been collected on NCAM Ig1-4 crystals belonging to space group P622 or P6₃22.

Rapid screening for HIV-1 protease inhibitor leads through X-ray diffraction

Hexagonal crystals of unliganded tethered HIV-1 protease combined with the soaking method for the preparation of crystalline complexes have been shown to be suitable for the rapid discovery of protease inhibitors as drugs against AIDS.

LySDB – Lysozyme Structural DataBase

LySDB is a centralized web resource with a user-friendly search engine developed to study the properties of the lysozyme structures available in the literature.

Microseed matrix screening to improve crystals of yeast cytosine deaminase

A crystallization strategy termed `microseed matrix screening' is described where the optimal conditions for nucleation versus extended lattice growth are not compatible.

Production and preliminary analysis of perdeuterated yeast inorganic pyrophosphatase crystals suitable for neutron diffraction

Perdeuterated yeast inorganic pyrophosphatase has been produced and crystallized. Neutron diffraction data have been recorded and the structure has been determined by X-ray diffraction.

Novel buffer systems for macromolecular crystallization

Broad-range buffer systems that consist of three component buffers can be substituted for a single buffer in crystallization trials, allowing pH and buffer type to be decoupled.

Soybean lipoxygenase-3 in complex with 4-nitrocatechol

The complex of lipoxygenase-3 with 4-nitrocatechol indicates His518 withdrawal from the iron coordination sphere and flexibility of the geometrical features of this site.

Comment on Flash-cooling of macromolecular crystals to overcome increased mosaicity by Yao, Yasutake & Tanaka (2004)

Comment on Yao et al. (2004), Acta Cryst. D60, 39–45.

Structural studies of Proteus mirabilis catalase in its ground state, oxidized state and in complex with formic acid. Erratum

An erratum to the paper by Andreoletti et al. (2003), Acta Cryst. D59, 2163–2168.

Solving the structure of the bubble protein using the anomalous sulfur signal from single-crystal in-house Cu Kα diffraction data only. Erratum

An erratum to the paper by Olsen et al. (2004), Acta Cryst. D60, 250–255.

The high-mosaicity illusion: revealing the true physical characteristics of macromolecular crystals. Erratum

A second erratum to the paper by Bellamy et al. Acta Cryst. (2000), D56, 986–995.

Structure of the saccharide-binding domain of the human natural killer cell inhibitory receptor p75/AIRM1. Erratum

An erratum to the paper by Dimasi et al. (2004), Acta Cryst. D60, 401–403.