research papers
Structures of the interacting domains from yeast glutamyl-tRNA synthetase and tRNA-aminoacylation and nuclear-export cofactor Arc1p reveal a novel function for an old fold
aStructural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany
*Correspondence e-mail: suck@embl.de
Eukaryotic aminoacyl-tRNA synthetases (aaRS) frequently contain additional appended domains that are absent from their prokaryotic counterparts which mediate complex formation between eukaryotic aaRS and cofactors of aminoacylation and translation. However, the structural basis of such interactions has remained elusive. The heteromerization domain of yeast glutamyl-tRNA synthetase (GluRS) has been cloned, expressed, purified and crystallized in C2221, with unit-cell parameters a = 52, b = 107, c = 168 Å. Phase information was obtained from multiple-wavelength with selenomethionine to 2.5 Å resolution and the structure, comprising two monomers per was determined and refined to 1.9 Å resolution. The structure of the interacting domain of its accessory protein Arc1p was determined and refined to 1.9 Å resolution in a crystal form containing 20 monomers organized in five tetramers per (space group C2, unit-cell parameters a = 222, b = 89, c = 127 Å, β = 99.4°). Both domains adopt a GST-like fold, demonstrating a novel role for this fold as a protein–protein interaction module.
1. Introduction
Aminoacyl-tRNA synthetases (aaRS) catalyze the highly specific charging of their cognate tRNAs with the corresponding amino acid. This reaction establishes the link between the anticodon, i.e. the nucleotide code, and the amino acid attached to the 3′ end of the i.e. the protein code. Therefore, aaRS may be considered to be the real translators of the In contrast to their prokaryotic counterparts, eukaryotic aaRS are integrated into an efficient nuclear-export and shuttling machinery and are interconnected closely with the translational apparatus. While prokaryotic aaRS typically function as monomers or dimers, eukaryotic enzymes frequently function as part of higher order complexes. This functional requirement is reflected by the presence of additional protein-binding and RNA-binding domains and a correspondingly higher degree of complex formation among eukaryotic aaRS (Mirande, 1991; Kisselev & Wolfson, 1994; Yang, 1996). The basic enzymatic function of prokaryotic and eukaryotic aaRS is structurally very well understood (Martinis et al., 1999; Woese et al., 2000) and a wealth of structural information is available for these enzymes. However, despite many reported interactions between eukaryotic aaRS and protein cofactors (Negrutskii et al., 1999; Quevillon et al., 1999; Kim et al., 2000; Deinert et al., 2001), a structural model of a protein–protein interaction involving a eukaryotic aaRS has not been reported.
In higher eukaryotes, nine aminoacyl-tRNA synthetases, including MetRS and GluProRS, associate to form a supramolecular multi-enzyme complex (Mirande et al., 1982; Kerjan et al., 1994; Lee et al., 2004). In addition to the enzymes, this complex contains three associated proteins, p18, p38 and p43 (Quevillon & Mirande, 1996; Quevillon et al., 1997; Kim et al., 2002). In the yeast Saccharomyces cerevisiae, an evolutionary intermediate aaRS complex is formed by MetRS, GluRS and the tRNA-aminoacylation and nuclear-export cofactor Arc1p, which shares sequence homology with all three accessory components of the multi-synthetase complex of higher eukaryotes (Simos et al., 1996). The yeast aaRS complex is a biochemically well characterized model system. Both synthetases and both tRNAGlu and tRNAMet can be efficiently copurified with protein A-tagged Arc1p from cellular yeast lysates (Simos et al., 1996). The N-terminal appended domains of the three proteins are necessary and sufficient for formation of the MetRS–Arc1p–GluRS ternary complex, which appears to be stoichiometric (Simos et al., 1998; Deinert et al., 2001; Galani et al., 2001). The interaction domain of Arc1p contains binding sites for MetRS and GluRS which enable it to bind both synthetases simultaneously and independently (Simos et al., 1998). The N-terminal appended interaction domains of the synthetases are non-catalytic and are absent in the prokaryotic counterparts. MetRS cannot interact with GluRS in the absence of Arc1p, demonstrating that the association is mediated by Arc1p (Simos et al., 1998; Galani et al., 2001).
We have chosen the N-terminally appended heteromerization domains of yeast glutamyl-tRNA synthetase (subsequently referred to as GluRS-N) and the tRNA-aminoacylation and nuclear-export cofactor Arc1p (subsequently referred to as Arc1p-N) as a well established model system to structurally characterize the two interacting domains.
2. Materials and methods
2.1. Cloning and expression of GluRS-N
Residues 1–197, 17–207 and 1–207 of S. cerevisiae cytoplasmic glutamyl-tRNA synthetase (EC 6.1.1.17; GeneBank accession P46655) were PCR-amplified from plasmid pEMBLyex4-His8-GluRS (Deinert et al., 2001) using primers Fw1 (TATAGAATTCCCGCTCCATATGACGAAACTATTTTCAAAGGTTAAGG), Fw17 (TATAGAATTCCCGCTCCATATGCCATCTACCTTGACTATTAATGG), Rv197 (CGCTCTAGATTACTTACCCACATTAGCACTTTTCTT) and Rv207 (CGCTCTAGATTATTCAAAGTTAGCCTTGTGAGTTTC). PCR products were restricted with FauI and XbaI (New England Biolabs) and ligated into an NdeI- and XbaI-restricted modified pETm (Novagen) vector. The expression construct allows the removal of the N-terminal six-His tag through TEV protease cleavage, leaving a Gly-His dipeptide N-terminal of the natural starting methionine. The sequence of the expression constructs was confirmed by sequencing with T7 primer. The constructs were transformed into Escherichia coli strain BL21(DE3) Star cells, which were grown at 310 K in 6 l TB medium containing 100 µg ml−1 carbenicillin (Sigma) to an OD600 of 0.6. Upon induction of recombinant protein expression with 0.1 mM IPTG, the incubation temperature was lowered to 291 K and the cells were grown for another 12 h.
2.2. Purification of GluRS-N and interaction analysis
Cells were harvested by centrifugation, resuspended in lysis buffer (50 mM Tris, 150 mM NaCl, 20 mM imidazole, 5 mM MgCl2, 10% glycerol, 10 mM β-mercaptoethanol, pH 7.5 with HCl) at 10 ml buffer per gram of wet cell paste and lysed with an Emulsiflex-C5 (Avestin, Canada). The lysate was cleared by centrifugation at 30 000g for 20 min and the supernatant was loaded onto nickel–nitrilotriacetic acid Superflow resin (Qiagen). The resin was washed with lysis buffer containing 1 M NaCl, followed by elution of the proteins with a linear gradient of 20–400 mM imidazole in lysis buffer. Protein peak fractions (typically >95% pure) were pooled, the buffer was exchanged to TEV protease buffer (50 mM Tris, 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 5 mM β-mercaptoethanol, pH 8.0 with HCl) using a Sephadex G-25 column and the sample was incubated with TEV protease in a 50:1 molar ratio at room temperature overnight for removal of the six-His tag. TEV protease, impurities and any remaining uncleaved six-His-tagged protein was removed by re-adsorption to nickel–nitrilotriacetic acid resin. The flowthrough was concentrated to 20 mg ml−1 using centrifugal filter devices (Centricon) and further purified on a 16/60 Superdex-75 column (Pharmacia) equilibrated and run in gel-filtration buffer (20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH 7.2 with NaOH). Pure peak fractions from the gel filtration were pooled, concentrated to 20 mg ml−1 and stored at 193 K for crystallization. Selenomethionine-substituted protein was expressed in E. coli B834 (DE3) and purified following the same basic protocol. Full-length GluRS is known to be monomeric in solution under physiological conditions (Deinert et al., 2001). The monomeric state of all three N-terminal fragments of GluRS was confirmed by dynamic and gel filtration. The ability of all three fragments to form a stable complex with Arc1p was confirmed by copurification with Arc1p in gel filtration on a 16/60 Superdex-200 column (results not shown). Arc1p was purified for this interaction analysis as described in Deinert et al. (2001).
2.3. Crystallization of GluRS-N
Crystallization trials were set up at 293 K in 96-well format using a Mosquito nanolitre pipetting robot (TTP Labtech). Sitting drops of 600 nl (1:1 mixture of 20 mg ml−1 protein in gel-filtration buffer and reservoir solution) were equilibrated by vapour diffusion against reservoirs containing 75 µl screen solution in Crystal Quick plates (Greiner Bio-One). 15 screens of 96 conditions each were tested with each GluRS fragment. While GluRS_1–197 and GluRS_17–207 did not yield crystalline material in any of the 1440 conditions tested, GluRS_1–207 crystallized under several closely related conditions from a commercial ammonium sulfate screen (Nextal). These crystals were optimized using microseeding in 24-well plates by vapour diffusion from hanging drops composed of equal volumes (typically 2 + 2 µl) of protein and reservoir solution equilibrated against 1 ml reservoir solution [1.7–1.8 M (NH4)2SO4, 200 mM KSCN for selenomethionine-substituted crystals and 1.8–1.9 M (NH4)2SO4, 200 mM NaI for native crystals]. The optimized condition yielded crystals suitable for X-ray analysis (see supplementary material1).
2.4. X-ray data collection
Crystals of GluRS-N were cryoprotected by sequential transfer to cryosolution [1.9 M (NH4)2SO4, 200 mM NaI or KSCN, 150 mM NaCl, 20 mM HEPES, 5 mM MgCl2, 1 mM DTT] with stepwise addition of glycerol to 15% prior to freezing in liquid nitrogen. Multiple-wavelength data were collected at 100 K from one selenomethionine-substituted crystal at the peak and inflection-point wavelength of selenium and at a high remote wavelength (Table 1) at the Swiss Light Source beamline PX1. Native data from a GluRS-N crystal frozen at 100 K were collected at the European Synchrotron Radiation Facility beamline ID-23. Native data from an Arc1p-N crystal frozen at 100 K were collected at the Swiss Light Source beamline PX1. Data reduction and scaling were performed with XDS. Data-collection statistics are given in Tables 1 and 2.
‡Anomalous signal-to-noise ratio as the mean σ(I) of acentric reflections assuming Friedel's law to be true divided by the mean σ(I) of accentric reflections assuming Friedel's law to be false. |
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2.5. Structure solution of GluRS-N
In order to solve the ) since was clearly not an option owing to the lack of any structures that were significantly related to GluRS-N by sequence homology. SHELXC (Sheldrick, 1990) was used to analyse and prepare the MAD data set for a heavy-atom site search using SHELXD. The heavy-atom site search with SHELXD (Sheldrick, 1990) on peak, inflection and high remote data sets in the resolution range 20–3.5 Å revealed six of the eight possible Se atoms in the of heavy-atom sites and calculation of the initial phases were performed with SOLVE (Terwilliger & Berendzen, 1999) and density modification and phase extension to the 2.5 Å high-resolution limit of the MAD data set were completed with RESOLVE (Terwilliger, 2000). The figure of merit for phasing was 0.76 for initial phases to 3.5 Å, 0.86 after density modification at 3.5 Å and 0.64 after extending the phases to 2.5 Å.
for this structure, we decided to attempt experimental phasing by the multiple-wavelength (MAD) method using selenomethionine-substituted crystals (Table 12.6. Model building and of GluRS-N
The structure was built manually in Coot (Emsley & Cowtan, 2004). During against the 1.9 Å native data set, 46 iodines were added to the structure based on difference-map analysis as NaI was absent from the crystallization solution of the selenomethionine-substituted crystals but was present at 200 mM in the crystallization solution of the native crystals. against the 1.9 Å native data set reached convergence (Table 3) after a few alternating cycles of model correction in Coot and allowing the anisotropic motion of rigid bodies, described as TLS parameters (Winn et al., 2001), as implemented in REFMAC5 (Murshudov et al., 1997). Water molecules were automatically added in Coot and the occupancy of the iodines was manually adjusted based on difference-map analysis. Inspection of the refined model with PROCHECK (Laskowski et al., 1993) revealed good stereochemistry (Table 3). The final refined model includes 189 amino-acid residues in both of the monomers in the Residues 1–16 and 197–207 of chain A and 1–13 and 194–207 of chain B were unidentified in the electron-density map and therefore molecular models for these segments are not present in the final model. Statistics for phasing and are summarized in Tables 2 and 3.
‡As calculated by PROCHECK (Laskowski et al., 1993). |
2.7. Phasing, model building and of Arc1p-N
Expression, purification, crystallization, X-ray ). While the initial map was not interpretable for model building, fivefold NCS averaging over the five tetramers present in the as implemented in RESOLVE (Terwilliger, 2000) subsequently produced a traceable map. The figure of merit for phasing by RESOLVE was 0.53 for initial phases to 3.5 Å, 0.65 after density modification at 3.5 Å and 0.59 after extending the phases to the 2.8 Å high-resolution limit of the selenomethionine MAD data set. The structure was built using the program O (Jones et al., 1991) by manually building one tetramer and then using MOLREP (Vagin & Teplyakov, 1997) to automatically place the remaining four tetramers of the into the phased electron-density map from RESOLVE. Strict NCS restraints between the five tetramers of the as implemented in REFMAC were maintained during the initial stages of and rebuilding in O (Jones et al., 1991). Ten sulfate molecules were located and identified by manual inspection of the difference electron-density maps and added manually. NCS restraints were progressively relaxed and replaced by TLS as implemented in REFMAC during the later stages of Coot was used for manual model correction and analysis during the later stages of and to automatically add water molecules. Inspection of the refined model with PROCHECK revealed excellent stereochemistry (Table 3). The 20 monomers of the final refined model differ somewhat in respect to the amino-acid residues that could be built in each chain. On average, 115 of 124 residues were built per monomer, with two to four residues missing from each terminus. Residues 14–18 from the first chain of each tetramer (corresponding to chains A, E, I, M and Q) as well as 16–22 from chain C, 22–31 from chain H and 19–21 from chain K could not be identified in the electron-density maps and these segments are therefore missing in the final model. Statistics for are summarized in Table 3.
and preliminary multiple-wavelength phasing of the Arc1p N-terminal heteromerization domain has been described in Simader & Suck (20062.8. Miscellaneous
PyMOL (DeLano, 2006) was used to render structural models for figure preparation. ClustalW (Thompson et al., 1994; Chenna et al., 2003) was used to calculate multiple sequence alignments and for phylogenetic distance analysis. Multiple sequence-alignment figures were prepared with ESPript (Gouet et al., 1999) applying the Risler scoring matrix (Risler et al., 1988) with a global similarity-score cutoff of 0.6, using consensus criteria from MULTALIN (Corpet, 1988) and using secondary-structure and accessibility information calculated using DSSP (Kabsch & Sander, 1983). The DALI server (Holm & Sander, 1993, 1998) was used to identify proteins that were structurally related to GluRS-N and Arc1p-N. We used automated error-scaled difference distance-matrix analysis of the 20 individual monomers contained in the of the Arc1p-N structure as implemented in ESCET (Schneider, 2000, 2002, 2004) in order to cluster the monomers by structural similarity at different σ levels and to distinguish between conformationally invariant and flexible regions of Arc1p-N. Clusters of highly similar conformers (with matrix elements larger than 0.98 at the 2σ level in ESCET) were reduced to a single representative with the minimal experimental error (mean e.s.d. in ESCET). The remaining five conformers were kept for analysis of invariant and flexible regions in ESCET. Protein interfaces and assemblies were analyzed using the protein interfaces, surfaces and assemblies service PISA at the European Bioinformatics Institute (Krissinel & Henrick, 2005).
3. Results and discussion
3.1. Structure of GluRS-N
The N-termini of Arc1p and GluRS were originally described as domains with little or no homology to known proteins (Simos et al., 1996, 1998). A relationship to glutathione S-transferase (GST) like proteins was subsequently predicted on the basis of conserved sequence motifs (Galani et al., 2001). Indeed, the of GluRS-N confirms that it adopts a GST-like fold (Fig. 1). The two monomers of GluRS-N contained in the are conformationally almost identical, as suggested by analysis with ESCET (Schneider, 2000, 2002, 2004; results not shown). The r.m.s.d. for superposition of GluRS-N chain B on chain A of E. coli GST (PDB code 1a0f; Nishida et al., 1998) is 2.04 Å for 145 Cα atoms with 17% sequence identity. However, the cleft formed between the N-terminal thioredoxin-like α/β motif (GST_N in PFAM; Bateman et al., 2004) and the C-terminal α-helical part of the classical GST fold (GST_C in PFAM), which is normally the substrate-binding cleft of enzymatically active GSTs, lacks residues suitable for substrate binding and does not bind to glutathione Sepharose (results not shown).
The open reading frame for S. cerevisiae GluRS encodes 16 N-terminal amino acids followed by a second methionine which are not contained in any homologue (Fig. 2). This has led to disagreement about which methionine represents the real start of translation; consequently, two alternative versions are found in the databases, one including the first 16 codons (e.g. SwissProt accession P46655) and one starting with the second methionine (e.g. the SGD entry for GluRS, GUS1). There does not appear to be any experimental evidence available to support one or the other. We have therefore tried to express, purify and crystallize different versions of GluRS-N with and without the nonconserved first 16 amino acids. While all three fragments could be expressed in soluble form, the levels of solubly expressed residues 17–207 were much lower than those of residues 1–197 and 1–207, suggesting that the first 16 amino acids may play a role in the folding or solubility of GluRS. However, neither residues 1–197 nor 17–207 could be crystallized, while crystals of residues 1–207 were readily obtained under several conditions from the initial screen, suggesting involvement of the first 16 and last ten amino acids in contacts. Surprisingly, the structural model of residues 1–207 reveals that the first 16 and the last 12 amino acids are not ordered in both monomers of the of this crystal form: there is hardly any interpretable electron density N-terminal of the second Met17 or C-terminal of Asn194 in either monomer. While it still remains unclear why the shorter constructs could not be crystallized, the structure demonstrates that the basic GST fold of the GluRS N-terminus is complete without the first 16 residues.
3.2. Unusual in the structure of Arc1p-N
The structure of Arc1p-N comprises 20 monomers organized into five tetramers per ). Given that Arc1p-N behaves as a monomer in solution (Deinert et al., 2001 and data not shown), the structure of Arc1p-N is outstanding in respect to its very high The five tetramers (Fig. 3) are almost identical to each other (the average r.m.s.d. for pairwise superpositions is 0.60 Å), but their arrangement within the is slightly irregular. They all differ in their relative orientation, with angular differences of between 4 and 14° in the xy plane of the (not shown), explaining why the data could not be indexed and processed in a with a smaller asymmetric unit.
(Fig. 3Within each tetramer, the individual monomers are related by pseudo-twofold rotational symmetry axes as indicated in Fig. 3(b). Two sulfates are found at a distance of about 18.5 Å in the central part of each tetramer. They are coordinated by four arginines (Arg54 from each of two monomers and Arg98 from each of the two other monomers of the tetramer) and two lysine side chains (Lys91 from the same two monomers that contribute Arg98; see supplementary material1). This arrangement suggests that the sulfates may play an important role in the stabilization of the tetramers by partially neutralizing the concentrated positive charges of the coordinating residues, which would otherwise create strong electrostatic repulsive forces that might prohibit tetramer formation. Interestingly, the arginines involved in this interaction are well conserved within most of the orthologous sequences (group 1 in Fig. 2b), suggesting a biological function for these residues. However, analysis of protein interfaces within each tetramer by PISA (not shown) also did not reveal any specific interactions within a tetramer that could result in the formation of stable quaternary structures. This is in accordance with the finding that Arc1p-N behaves as a monomer in solution (Deinert et al., 2001 and results not shown). Thus, the arrangement of Arc1p-N monomers into tetramers as observed in our structure is most likely to be an artefact of crystallization.
3.3. Alternative conformations of the N-terminal 22 residues in Arc1p-N are determined by crystal-packing constraints
Clustering of the Arc1p-N monomers by structural variance at the 3σ significance level in ESCET (Schneider, 2000, 2002, 2004) reveals three major conformational variants corresponding to the three alternative conformations of the N-terminal 22 residues as suggested by manual analysis. The different conformations are adopted by corresponding monomers within each tetramer: conformation 1 (brown monomer in Figs. 3b and 4) is adopted by the first chain of each tetramer (chains A, E, I, M, Q), conformation 2 (green monomer in Figs. 3b and 4) is adopted by the second chain (chains B, F, J, N, R) and conformation 3 (grey monomers in Figs. 3b and 4) is adopted by the third and fourth chain of each tetramer (chains C, D, G, H, K, L, O, P, S, T). Analysis of the crystal-packing environment of this N-terminal segment reveals why it adopts three different conformations and suggests that conformer 3 represents the default conformation which is adopted in the absence of packing constraints. In conformers 1 and 2, this segment is deeply involved in crystal contacts between neighbouring tetramers stacking on top of each other along the z axis (Fig. 4a). Both conformations 1 and 2 in fact represent a `mini' domain swap of the N-terminal segment with that of the corresponding monomer from the next tetramer (Figs. 4b and 4c). In the case of conformer 1 (Fig. 4b), this domain swap is not clearly defined in our structure as the residues connecting the domain-swapped N-terminal segment to the remainder of the monomer are not ordered and could not be built for any of the five tetramers in the However, the Fo − Fc and 2Fo − Fc maps suggest this connectivity rather than that corresponding to conformer 3 and superposition of the putative `default' conformer 3 (green and yellow Cα traces in Figs. 4b and 4c) on the two domain-swapped conformer 1 monomers (brown and light brown in Fig. 4b) reveals that the crystal packing is sterically incompatible with conformation 3 in this region (clashes between the yellow and green Cα traces in Fig. 4b). In the case of conformer 2 (Fig. 4c), this domain swap is clearly defined in the structure by continous electron density at the 1.7σ level of the 2Fo − Fc map for all connecting residues in all five monomers corresponding to this conformer. As for conformer 1, superposition of the putative `default' conformer 3 (green and yellow Cα traces in Figs. 4b and 4c) on the two domain-swapped conformer 2 monomers (green and light green in Fig. 4c) reveals that the crystal packing is sterically incompatible with conformation 3 in place of conformation 2 (clashes between the yellow and green Cα traces in Fig. 4b). Thus, the alternative conformations observed for the N-terminal 22 amino acids correspond to two different ways of domain-swapping of the first α-helix of two Arc1p-N monomers and are fully explained by crystal-packing constraints.
3.4. Structure of the Arc1p-N monomer
As for GluRS-N, the ). Weak similarity to GSTs is reflected by the r.m.s.d. for superposition of Arc1p1-N chain D on chain A of E. coli GST (PDB code 1a0f; Nishida et al., 1998), which is 2.71 Å for 92 Cα atoms at 18.5% sequence identity. In contrast to GluRS-N, the Arc1p N-terminal domain corresponds to only the C-terminal α-helical part of the classical GST fold (GST_C in PFAM; Bateman et al., 2004) and thus lacks the N-terminal α/β motif, which contributes most of the substrate-binding residues in classical GSTs. As a consequence, the substrate-binding cleft of classical GSTs is missing and Arc1p cannot be enzymatically active as a GST. The multiple sequence alignment of Arc1p-N with orthologous sequences from other yeasts (Fig. 2b) suggests that the N-terminal α/β motif is still contained in the sequences from the phylogenetic group 2 (Ustilago maydis and Schizosaccharomyces pombe). This is supported by secondary-structure prediction, fold-recognition and threading approaches (results not shown), suggesting that the truncation of the Arc1p-N fold occurred in the time between the phylogenetic separation of groups 1 and 2.
of Arc1p-N confirms that the isolated monomer adopts a fold similar to the GST fold (Fig. 13.5. Possible interactions between GluRS-N and Arc1p-N
Arc1p-N and GluRS-N are known to be necessary and sufficient for formation of the yeast aaRS complex in vivo and in vitro (Simos et al., 1998; Deinert et al., 2001; Galani et al., 2001) and the ability of our Arc1p-N and GluRS-N proteins to form a stable complex with each other under near-physiological conditions was confirmed by copurification in gel filtration (results not shown). On the basis of the three-dimensional structures of the two isolated domains, we can now speculate about how the two domains might associate to form a binary complex.
Both domains turned out to adopt a GST-like fold. Classical GSTs form homodimers and interact in a characteristic way that relates the monomers by twofold rotational symmetry. Therefore, Arc1p-N and GluRS-N may be expected to interact in a corresponding mode (Fig. 5a). However, this interaction has so far been described as a homomeric interaction only. Alternatively, GluRS-N and Arc1p-N may interact in a manner suggested by the crystal-packing contacts within a tetramer of the Arc1p-N structure (Fig. 5a). Superposition of GluRS-N onto a monomer of Arc1p-N within a tetramer reveals that the most closely matching segments are those involved in contacts between monomers of Arc1p within a tetramer. This is particularly true for the horizontal interaction shown in Fig. 5(a), suggesting that GluRS and Arc1p might dock onto each other via this interface. The three possible interactions of GluRS-N with Arc1p-N, representing (i) the classical GST homodimerization and (ii) and (iii) the two different intermonomer contacts observed in each tetramer of Arc1p-N, would involve three distinct non-overlapping surface regions of GluRS-N.
N-terminal appendices homologous to Arc1p-N and GluRS-N are conserved in orthologous GluRS and Arc1p proteins from other yeast species (Fig. 2) and the only known biological function of this domains is to mediate complex formation between the proteins. The interfacing residues would therefore be expected to be the most conserved feature of the orthologous sequences. We therefore analysed the GluRS-N and Arc1p-N surface for patches of conserved residues in order to test the agreement between putative conserved surface patches and candidate interface regions. However, mapping of the conservation scores derived from the multiple sequence alignment of GluRS-N (Fig. 2) onto the surface of GluRS-N did not reveal a significant difference in surface conservation between the three putative interfacing surface regions (Fig. 5b). The result for Arc1p-N is similar (not shown), with the additional complication that Arc1p-N is known to harbour two independent interfaces for GluRS and MetRS (Simos et al., 1998; Deinert et al., 2001; Galani et al., 2001). We have analysed the electrostatic of both proteins in a similar way (not shown), but could not identify any strong complementary electrostatic patches for the candidate interface surfaces. Whatever the real interface is, the contacting residues appear on average to be no more conserved than other surface residues not involved in the interaction. This unexpected finding raises the question whether GluRS-N and Arc1p-N may have additional previously unrecognized functions resulting in surface conservation in addition to the interfacing surface and suggests a high plasticity of the interface.
Supporting information
Supporting information file. DOI: https://doi.org/10.1107/S0907444906039850/hv5066sup1.pdf
Acknowledgements
The authors are grateful to J. Basquin and A. Scholz (EMBL Heidelberg) for crystallization screen setup and would like to thank the beamline staff at the European Synchrotron Radiation Facility (Grenoble, France) ID-23 and Swiss Light Source (Villigen, Switzerland) PX-1 for their assistance during data collection. MH gratefully acknowledges financial support from the Peter and Traudl Engelhorn Foundation, Penzberg, Germany. This work is supported by the EU grant 3D Repertoire, contract No. LSHG-CT-2005-512028.
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