Numerology

Structure of the human p53 core domain in the absence of DNA

The crystal structure of the human p53 core domain in the absence of DNA has been determined with a new monoclinic space group (P2₁). Detailed analysis of non-crystallographic symmetry and crystal packing reveals that the p53 molecules are arranged differently from those in other p53 crystals, suggesting the possible existence of different p53 core domain tetramers.

Structure-based hypothesis on the activation of the CO-sensing transcription factor CooA

The structure of a CooA variant from the thermophilic bacterium Carboxydothermus hydrogenoformans (ChCooA) is reported in which one monomer is fully in the on-state.

Structure of glutaredoxin Grx1p C30S mutant from yeast

The structures of S. cerevisae glutaredoxin 1 and of a fusion protein consisting of yellow fluorescent protein and glutaredoxin were solved by molecular replacement and refined to 2.0 and 2.7 Å, respectively.

His-tag impact on structure

Crystal structures with and without His-tags have been surveyed and the authors show that His-tags are not hazardous to your structure.

Reduction of X-ray-induced radiation damage of macromolecular crystals by data collection at 15 K: a systematic study

A small but significant reduction of X-ray-induced radiation damage at 15 K compared with 90 K could be found in the systematic study of a total of 54 crystals of insulin and holoferritin.

A novel strategy for the crystallization of proteins: X-ray diffraction validation

Difference Fourier X-ray diffraction analyses of nine crystals are presented, which convincingly demonstrate the validity of the hypothesis that protein crystallization can be driven by the inclusion of small molecules rich in hydrogen-bonding, hydrophobic and electrostatic bonding possibilities.

Nonstoichiometric complex of gramicidin D with KI at 0.80 Å resolution

Structure of double-stranded right-handed double-helical gramicidin channels (dimers) in the crystal of gramicidin D with KI complex has been determined. High-resolution data enabled quantitative determination of gramicidin components and distributions of the I anions outside and K cations inside the channel, which was confirmed by their anomalous scattering.

Studies of Toxoplasma gondii and Plasmodium falciparum enoyl acyl carrier protein reductase and implications for the development of antiparasitic agents

The crystal structures of T. gondii and P. falciparum ENR in complex with NAD⁺ and triclosan and of T. gondii ENR in an apo form have been solved to 2.6, 2.2 and 2.8 Å, respectively.

A methodology and an instrument for the temperature-controlled optimization of crystal growth

A methodology and an instrument for the temperature-controlled optimization of crystal growth are described. The technique finds application in the growth of large high-quality crystals for neutron crystallography.

An extremely SAD case: structure of a putative redox-enzyme maturation protein from Archaeoglobus fulgidus at 3.4 Å resolution

The SAD technique was used to determine the crystal structure of a putative redox-enzyme maturation protein from A. fulgidus at 3.4 Å resolution.

Structure of archaeal glyoxylate reductase from Pyrococcus horikoshii OT3 complexed with nicotinamide adenine dinucleotide phosphate

The crystal structure of glyoxylate reductase from the hyperthermophilic archaeon P. horikoshii OT3 complexed with NADP(H) was solved at 1.7 Å resolution. The present study revealed its cofactor-recognition and thermostability mechanisms.

On the routine use of soft X-rays in macromolecular crystallography. Part IV. Efficient determination of anomalous substructures in biomacromolecules using longer X-ray wavelengths

The anomalously scattering substructures in 23 crystals of 19 different biological macromolecules have been determined based on diffraction data collected at a wavelength of 2.0 Å.

Structural asymmetry and intersubunit communication in muscle creatine kinase

The structure of a transition-state analog of muscle creatine kinase reveals significant asymmetry within the functional homodimer. The amino-terminal region is shown to be intimately involved in subunit association and intersubunit communication.

Structure of the AlaX-M trans-editing enzyme from Pyrococcus horikoshii

The crystal structure of the AlaX-M trans-editing enzyme revealed that the conserved glycine-rich loop in the N-terminal domain is located near the catalytic site in the C-terminal domain.

Double-stranded helical twisted β-sheet channels in crystals of gramicidin S grown in the presence of tri­fluoro­acetic and hydrochloric acids

Gramicidin S, a membrane-active antibiotic, was crystallized from solvent containing water, methanol, trifluoroacetic acid and hydrochloric acid. The structure was refined at 0.95 Å resolution and contains 1.5 molecules of gramicidin S, two trifluoracetic acid molecules and ten water molecules in the crystallographic asymmetric unit. In the crystals, the gramicidin S molecules line up into helical channels that differ from those observed previously.

Structures of two mutants that probe the role in iron release of the dilysine pair in the N-lobe of human transferrin

The crystal structures of two mutants of the N-terminal half-molecule of human transferrin, K206E and K206E/K296E, have identified important features of the mechanism of iron release.

Slow diffusion of lactose out of galectin-3 crystals monitored by X-ray crystallography: possible implications for ligand-exchange protocols

Structures of human galectin-3 carbohydrate-recognition domain determined at various stages of ligand removal provided insight into ligand-diffusion rates and their significance with respect to ligand-exchange protocols.

PET Chemistry. The Driving Force in Molecular Imaging

Deposition of structural data redux

Notes for authors 2007