Neutron and X-ray single-crystal diffraction from protein microcrystals via magnetically oriented microcrystal arrays in gels

Single-crystal neutron and X-ray diffraction from lysozyme microcrystals magnetically aligned in gels are reported. Neutron diffraction spots were observed to a resolution of 3.4 Å and the crystal structure was solved at a resolution of 1.76 Å using X-rays at room temperature.

Ab initio phasing by molecular averaging in real space with new criteria: application to structure determination of a betanodavirus

A two-step process of phase determination in the X-ray structural analysis of the coat protein of a betanodavirus is described. A new indicator, the free fraction, for molecular averaging in real space is introduced to effectively evaluate the phasing power in order to enhance the success of determining new structures.

Structural basis for acyl-group discrimination by human Gcn5L2

Structures of human Gcn5L2 bound to propionyl-CoA and butyryl-CoA show how the active site accommodates different acyl modifications and explain why butyryl-CoA acts as a competitive inhibitor.

Second harmonic generation correlation spectroscopy for characterizing translationally diffusing protein nanocrystals

Second harmonic generation correlation spectroscopy was developed as a tool for the characterization of diffusing protein nanocrystals.

Structural and functional studies of the glycoside hydrolase family 3 β-glucosidase Cel3A from the moderately thermophilic fungus Rasamsonia emersonii

Cel3A from the thermophilic fungus R. emersonii has proven to be more efficient in the hydrolysis of β-glycosidic linkages than Cel3A from H. jecorina.

Angular-split/temporal-delay approach to ultrafast protein dynamics at XFELs

An angular-split/temporal-delay approach offers a solution to crystallographic observations of ultrafast structural dynamics in biological macromolecules.

Mechanism of the allosteric regulation of Streptococcus mutans 2′-deoxycytidylate deaminase

The allosteric regulation of S. mutans 2′-deoxycytidylate deaminase by dCTP/dTTP is based on a concentration-dependent competition mechanism. This is the first pair of dTTP/dCTP-bound crystal structures of deoxycytidylate deaminase from the same species to be reported.

Visualizing the Bohr effect in hemoglobin: neutron structure of equine cyanomethemoglobin in the R state and comparison with human deoxyhemoglobin in the T state

The determination of the positions of H/D atoms in equine cyanomethemoglobin by neutron diffraction is described.