Findable Accessible Interoperable Re-usable (FAIR) diffraction data are coming to protein crystallography

The policy of IUCr Journals on diffraction data is defined.

Design guidelines for an electron diffractometer for structural chemistry and structural biology

Installation of the EIGER X 1M detector onto an electron microscope and system calibration for data collection turns a transmission electron microscope into an electron diffraction instrument. Data can be collected and processed with a throughput that meets the requirements of a modern X-ray facility. The setup described here offers access to single-crystal structure determination from microcrystalline powder when large single crystals cannot be produced.

Visualizing membrane trafficking through the electron microscope: cryo-tomography of coat complexes

The study of coat protein assemblies on membranes by cryo-electron tomography and subtomogram averaging is discussed, outlining the methodological advancements that enabled the gain of crucial structural insight.

Structural and functional insights into phosphomannose isomerase: the role of zinc and catalytic residues

Phosphomannose isomerase is a zinc-dependent enzyme involved in reversible isomerization of mannose 6-phosphate and fructose 6-phosphate. Based on extensive site-directed mutagenesis, structural and biochemical studies on phosphomannose isomerase from Salmonella typhimurium, zinc ions were found to be indispensable for substrate binding and the catalytic base was identified as Lys86.

Crystal structure of the leucine-rich repeat ectodomain of the plant immune receptor kinase SOBIR1

The ectodomain structure of a novel plant membrane receptor kinase with unusual capping domains is reported.

Structural analysis of a replication protein encoded by a plasmid isolated from a multiple sclerosis patient

The crystal structure of the WH1 domain of the eukaryotic replication initiator protein (Rep) MSBI1.176 in the dimeric form has been determined to 1.53 Å resolution and shows a number of structural similarities to and differences from other known prokaryotic Reps.

Analysis and validation of macromolecular B values

This paper describes some statistical tools for analyses of macromolecular B values.

Protein Tyrosine Phosphatases. Structure, Signaling and Drug Discovery. Edited by L. G. Ahuja. De Gruyter, 2018. Pp. 282. Price (hardback) 139.95 EUR, 160.99 USD, 127 GBP. ISBN 978-3-11-042643-4.

Sir Aaron Klug OM FRS (1926–2018)

Nobel Prize winner Sir Aaron Klug is remembered.