Methods for merging data sets in electron cryo-microscopy

A workflow to combine cryo-EM data collected at different magnification.

Relationship between the induced-fit loop and the activity of Klebsiella pneumoniae pullulanase

The induced-fit motion of an active-site loop (residues 706–710) including the catalytic acid/base residue Glu706 in pullulanase from Klebsiella pneumoniae (KPP) belonging to glycoside hydrolase family 13 subfamily 13 was examined by mutational and structural analysis. Analysis of the structure and activity of recombinant pullulanase from K. pneumoniae ATCC 9621 (rKPP) and its G680L mutant indicated that the side chain of residue 680 is important for the conformational change of the loop 706–710 in the ligand-free form, resulting in the altered binding affinity of the substrate.

The subatomic resolution study of laccase inhibition by chloride and fluoride anions using single-crystal serial crystallography: insights into the enzymatic reaction mechanism

A study of laccase inhibition by chloride and fluoride anions is presented. Single-crystal serial crystallography at subatomic resolution is applied to a study of the enzymatic reaction mechanism.

Calcium-ligand variants of the myocilin olfactomedin propeller selected from invertebrate phyla reveal cross-talk with N-terminal blade and surface helices

By installing calcium-ligand triads found in olfactomedin sequences from invertebrates, insight was gained into calcium-binding features across the protein family and new surface conformations were observed that may be relevant to elusive protein–protein interactions.

Crystal structure of the N-terminal domain of the major virulence factor BB0323 from the Lyme disease agent Borrelia burgdorferi

The crystal structure of the N-terminal domain of the major virulence factor BB0323 from the Lyme disease agent Borrelia burgdorferi, which is essential for membrane integrity, cell fission and infectivity, is reported.

Structures of the substrate-binding protein YfeA in apo and zinc-reconstituted holo forms

A cluster A-I substrate-binding protein reveals conformational changes, including an asymmetric rigid-body rotation of the flexible lobe, the reordering of a mobile helix and a spring-hammer mechanism.

X-ray structure of the direct electron transfer-type FAD glucose de­hydrogenase catalytic subunit complexed with a hitchhiker protein

The X-ray structure of the catalytic subunit of Burkholderia cepacia FAD glucose dehydrogenase complexed with a hitchhiker protein was determined as a representative molecule of direct electron transfer-type FAD-dependent dehydrogenase complexes. The 3Fe–4S cluster is present at the surface of the catalytic subunit and serves in the intramolecular and intermolecular electron transfer from FAD to the electron-transfer subunit.

Supercell refinement: a cautionary tale

A higher dimensional superspace description accounts for an unexpected supercell refinement result.