Crystal structure of an N^ω-hy­droxy-L-arginine hydrolase found in the D-cycloserine biosynthetic pathway

The crystal structure of DcsB, an N^ω-hydroxy-L-arginine hydrolase found in the D-cycloserine biosynthetic pathway, is reported.

Inactive dimeric structure of the protease domain of stomatin operon partner protein

Based on the structure of the N-terminal region of the S97A mutant of the stomatin operon partner protein PH1510 (1510-N), it was found that the dimeric form differs greatly from those determined previously. This result indicates that 1510-N can greatly change the form of its dimer.

Crown-ether-mediated crystal structures of the glycosyltransferase PaGT3 from Phytolacca americana

Crystal structures of the glycosyltransferase PaGT3 crystallized in the presence of crown ethers provide a basis for understanding its acceptor-recognition mechanism.

Current approaches for automated model building into cryo-EM maps using Buccaneer with CCP-EM

The model-building software Buccaneer has been repurposed based on existing methods for building models into cryo-EM maps. These approaches are implemented in the latest CCP-EM model-building pipeline (version 1.4.0).

Structural studies of geranylgeranylglyceryl phosphate synthase, a prenyltransferase found in thermophilic Euryarchaeota

The X-ray structure of geranylgeranylglyceryl phosphate synthase from Thermoplasma volcanium is reported, and a phylogenetic analysis and parallel ancestral state reconstruction of the evolution of the enzyme are reported for the phylum Euryarchaeota.

High-resolution crystal structure of Trypanosoma brucei pteridine reductase 1 in complex with an innovative tricyclic-based inhibitor

Structural insights into the development of a tricyclic compound as an inhibitor of Trypanosoma brucei pteridine reductase 1 are reported.

A baculoviral system for the production of human β-glucocerebrosidase enables atomic resolution analysis

Active human β-glucocerebrosidase was produced in baculovirus-transduced insect cells, purified and crystallized to yield a 0.98 Å resolution unliganded structure and a trapped covalent intermediate structure with 2-deoxy-2-fluoro-β-D-glucopyranoside.

Structural analysis of the PATZ1 BTB domain homodimer

The crystal structures of the PATZ1 BTB domains from mammals and fish contain homodimers. The core dimer conformation of these BTB proteins is dynamically stable, despite the presence of highly flexible regions in the dimerization interface.

Structural basis of the dominant inheritance of hyperme­thioninemia associated with the Arg264His mutation in the MAT1A gene

Dominant inheritance of hypermethioninemia is caused by an Arg264His mutation in methionine adenosyltransferase. The mutation lowers the affinity for dimer–dimer interaction and enzymatic activity, which can be restored by chemical intervention.

Jean-Luc Ferrer (1964–2020): structural biologist, beamline instrumentation innovator and entrepreneur

An obituary is published for Jean-Luc Ferrer, an internationally known and highly respected scientist in protein crystallography, whose work led to many innovative instrumental and methodological developments in structural biology.