CsMT: a robust and streamlined CryoSPARC workflow for cryo-EM reconstruction of microtubules

CsMT is a robust and streamlined CryoSPARC-based workflow to reconstruct undecorated and decorated microtubule maps. By unifying the processing pipeline into a single user-friendly tool, it makes high-resolution microtubule research significantly more accessible and reproducible for laboratories worldwide.

Beyond monofunctionality: structural perspectives on phenol oxidase side activity in a catalase

Structural and biochemical characterization of the extracellular fungal catalase ClCPO uncovers an unexpected phenol oxidase promiscuity. Crystal structures, substrate docking and electron-transfer pathway analysis identify ligand-binding regions and support a working mechanistic model for catalase-associated phenol oxidase activity.

3dcon: tomogram denoising by deconvolution

An open-source entropy-regularized deconvolution workflow is presented for denoising tomographic reconstructions from cryo-electron microscopy.

Experiences developing structural biology tools with AI coding assistance

Artificial intelligence (AI)-assisted development can significantly reduce the effort of coding scientific tools and software. At the same time, the approach requires detailed oversight, as well as domain knowledge and codebase expertise. The conditions under which AI assistance is reliable, the kinds of mistakes coding LLMs can make and why expert judgment is necessary are examined through the example of developing a new CIF file parser that focuses particularly on speed of execution.

Crystal structures of SARS-CoV-2 main protease and its complex with PBI-0451

Crystal structures of SARS-CoV-2 main protease (M^pro) and its complex with PBI-0451 expand the conformational landscape of M^pro and offer structural guidance for the design of M^pro-targeted antiviral drugs.

Structural identification of endogenous Escherichia coli proteins from heterogeneous cryo-electron microscopy datasets

Cryo-EM analysis of a size-exclusion chromatography fraction initially attributed to an oligomeric form of a recombinant OGFOD1 protein expressed in Escherichia coli instead revealed three endogenous E. coli proteins: ArnA, GTP cyclohydrolase I and glycerol dehydrogenase. The three protein assemblies had comparable predicted hydrodynamic radii, which plausibly explained their partial coelution during size-exclusion chromatography, indicating that rigid, symmetric endogenous complexes can dominate heterogeneous cryo-EM datasets and highlighting the value of systematic analysis of off-target particle populations.

Defining the conformational landscape of glycogen synthase kinase 3 beta (GSK3β)

This article presents a systematic structural curation of all deposited GSK3β crystal structures to map its conformational ensemble, revealing eight distinct conformational states.

Fragment-based active-site exploration of urethane hydrolases reveals a diversity of urethane binding modes

Fragment-based active-site exploration of urethanases was performed to elucidate polyurethane binding and cleavage.

Protein structure of a glycoside hydrolase family 30, subfamily 12 endo-1,4-β-xylanase. Corrigendum

The article by St John et al. [(2026), Acta Cryst. D82, 370–382] is corrected.