organic compounds
Methyl 3-O-α-L-fucopyranosyl β-D-glucopyranoside tetrahydrate
aDepartment of Material and Environmental Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden, and bDepartment of Organic Chemistry, Arrhenius Laboratory, Stockholm University, SE-106 91 Stockholm, Sweden
*Correspondence e-mail: lars.eriksson@mmk.su.se
The title compound, C13H24O10·4H2O, is the methyl glycoside of a disaccharide structural element present in the backbone of the capsular polysaccharide from Klebsiella K1, which contains only three sugars and a substituent in the polysaccharide repeating unit. The conformation of the title disaccharide is described by the glycosidic torsion angles φH = 51.1 (1)° and ψH = 25.8 (1)°. In the crystal, a number of O—H⋯O hydrogen bonds link the methyl glycoside and water molecules, forming a three-dimensional network. One water molecule is disordered over two positions with occupancies of 0.748 (4) and 0.252 (4).
Related literature
For a background to capsular et al. (1988); Erbing et al. (1976); Gloaguen et al. (1999); Cescutti et al. (2005). For details of the puckering analysis, see: Cremer & Pople (1975). For the synthesis, see: Baumann et al. (1988). For a related structure, see: Eriksson & Widmalm (2012).
(CPS), see: JanssonExperimental
Crystal data
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Refinement
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Data collection: CrysAlis CCD (Oxford Diffraction, 2008); cell CrysAlis CCD; data reduction: CrysAlis RED (Oxford Diffraction, 2008); program(s) used to solve structure: SHELXS97 (Sheldrick, 2008); program(s) used to refine structure: SHELXL97 (Sheldrick, 2008); molecular graphics: DIAMOND (Brandenburg, 1999); software used to prepare material for publication: PLATON (Spek, 2009).
Supporting information
10.1107/S1600536812041992/is5198sup1.cif
contains datablocks I, global. DOI:Structure factors: contains datablock I. DOI: 10.1107/S1600536812041992/is5198Isup2.hkl
The synthesis of the title compound was described by Baumann et al. (1988) in which the fucose and glucose residues have the L and D absolute configurations, respectively. The compound was crystallized by slow evaporation of a mixture of water and ethanol (1:1) at ambient temperature.
All hydrogen atoms, except those on the water molecules, were geometrically placed and constrained to ride on the parent atom. The C—H bond distances were set to 0.98 Å for CH3, 0.99 Å for CH2, 1.00 Å for CH. The O—H bond distance was set to 0.84 Å for OH groups. The Uiso(H) = 1.5 Ueq(C, O) for the CH3 and OH, while it was set to 1.2 Ueq(C) for all other H atoms. One of the water positions, OW4 was disordered over two positions with the occupancy 0.748 (4) for OW4A and 0.252 (4) for OW4B. Using the non-merged dataset for
the refined to x = 0.0 but the s.u. was estimated to 0.3. This low accuracy of x is a result of the absence of significant effects, thus the value of the was not considered as meaningful, and the 2677 Friedel equivalents were included in the merging process (MERG 4 in SHELXL) for the final The of each sugar residue is known from the starting compounds used in the synthesis. The hydrogen atoms of the water molecule were located from difference density map, given Uiso(H) = 1.5Ueq(O) and in the the d(O—H) and d(H..H) were restrained to retain the previously known geometry of the water molecule.Data collection: CrysAlis CCD (Oxford Diffraction, 2008); cell
CrysAlis CCD (Oxford Diffraction, 2008); data reduction: CrysAlis RED (Oxford Diffraction, 2008); program(s) used to solve structure: SHELXS97 (Sheldrick, 2008); program(s) used to refine structure: SHELXL97 (Sheldrick, 2008); molecular graphics: DIAMOND (Brandenburg, 1999); software used to prepare material for publication: PLATON (Spek, 2009).Fig. 1. A view of the molecule with atom numbering scheme. The displacement ellipsoids are drawn at the 50% probability level for non-H atoms. |
C13H24O10·4H2O | F(000) = 444 |
Mr = 412.39 | Dx = 1.451 Mg m−3 |
Monoclinic, P21 | Mo Kα radiation, λ = 0.71073 Å |
Hall symbol: P 2yb | Cell parameters from 26696 reflections |
a = 9.6150 (2) Å | θ = 3.9–41.0° |
b = 7.1362 (1) Å | µ = 0.13 mm−1 |
c = 13.9716 (2) Å | T = 100 K |
β = 100.1180 (18)° | Prism, colourless |
V = 943.75 (3) Å3 | 0.15 × 0.05 × 0.03 mm |
Z = 2 |
Oxford Xcalibur 3 diffractometer with Sapphire 3 CCD | 4836 independent reflections |
Radiation source: Enhance (Mo) X-ray Source | 4644 reflections with I > 2σ(I) |
Graphite monochromator | Rint = 0.030 |
Detector resolution: 16.5467 pixels mm-1 | θmax = 36.3°, θmin = 4.0° |
ω scans at different ϕ | h = −16→16 |
Absorption correction: multi-scan (CrysAlis RED; Oxford Diffraction, 2008) | k = −11→6 |
Tmin = 0.976, Tmax = 0.996 | l = −23→23 |
37858 measured reflections |
Refinement on F2 | Secondary atom site location: difference Fourier map |
Least-squares matrix: full | Hydrogen site location: inferred from neighbouring sites |
R[F2 > 2σ(F2)] = 0.028 | H atoms treated by a mixture of independent and constrained refinement |
wR(F2) = 0.073 | w = 1/[σ2(Fo2) + (0.0456P)2 + 0.0842P] where P = (Fo2 + 2Fc2)/3 |
S = 1.08 | (Δ/σ)max < 0.001 |
4836 reflections | Δρmax = 0.44 e Å−3 |
294 parameters | Δρmin = −0.26 e Å−3 |
16 restraints | Extinction correction: SHELXL, Fc*=kFc[1+0.001xFc2λ3/sin(2θ)]-1/4 |
Primary atom site location: structure-invariant direct methods | Extinction coefficient: 0.020 (4) |
C13H24O10·4H2O | V = 943.75 (3) Å3 |
Mr = 412.39 | Z = 2 |
Monoclinic, P21 | Mo Kα radiation |
a = 9.6150 (2) Å | µ = 0.13 mm−1 |
b = 7.1362 (1) Å | T = 100 K |
c = 13.9716 (2) Å | 0.15 × 0.05 × 0.03 mm |
β = 100.1180 (18)° |
Oxford Xcalibur 3 diffractometer with Sapphire 3 CCD | 4836 independent reflections |
Absorption correction: multi-scan (CrysAlis RED; Oxford Diffraction, 2008) | 4644 reflections with I > 2σ(I) |
Tmin = 0.976, Tmax = 0.996 | Rint = 0.030 |
37858 measured reflections |
R[F2 > 2σ(F2)] = 0.028 | 16 restraints |
wR(F2) = 0.073 | H atoms treated by a mixture of independent and constrained refinement |
S = 1.08 | Δρmax = 0.44 e Å−3 |
4836 reflections | Δρmin = −0.26 e Å−3 |
294 parameters |
Geometry. All e.s.d.'s (except the e.s.d. in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell e.s.d.'s are taken into account individually in the estimation of e.s.d.'s in distances, angles and torsion angles; correlations between e.s.d.'s in cell parameters are only used when they are defined by crystal symmetry. An approximate (isotropic) treatment of cell e.s.d.'s is used for estimating e.s.d.'s involving l.s. planes. |
Refinement. Refinement of F2 against ALL reflections. The weighted R-factor wR and goodness of fit S are based on F2, conventional R-factors R are based on F, with F set to zero for negative F2. The threshold expression of F2 > σ(F2) is used only for calculating R-factors(gt) etc. and is not relevant to the choice of reflections for refinement. R-factors based on F2 are statistically about twice as large as those based on F, and R- factors based on ALL data will be even larger. |
x | y | z | Uiso*/Ueq | Occ. (<1) | |
C1F | 0.06137 (8) | 0.74750 (12) | 0.36039 (6) | 0.00779 (13) | |
H1F | −0.0206 | 0.8096 | 0.3826 | 0.009* | |
C2F | 0.12838 (8) | 0.60944 (12) | 0.43883 (6) | 0.00787 (13) | |
H2F | 0.1488 | 0.6794 | 0.5017 | 0.009* | |
C3F | 0.26863 (8) | 0.53512 (12) | 0.41753 (6) | 0.00798 (13) | |
H3F | 0.2504 | 0.4603 | 0.3561 | 0.010* | |
C4F | 0.36573 (8) | 0.69748 (13) | 0.40472 (6) | 0.00811 (13) | |
H4F | 0.4554 | 0.6474 | 0.3877 | 0.010* | |
C5F | 0.29166 (9) | 0.81952 (13) | 0.32155 (6) | 0.00935 (13) | |
H5F | 0.2719 | 0.7426 | 0.2608 | 0.011* | |
O5F | 0.15932 (7) | 0.88703 (10) | 0.34442 (5) | 0.00936 (11) | |
C6F | 0.37691 (10) | 0.98940 (15) | 0.30347 (7) | 0.01461 (16) | |
H6FA | 0.3243 | 1.0623 | 0.2495 | 0.022* | |
H6FB | 0.4672 | 0.9491 | 0.2869 | 0.022* | |
H6FC | 0.3945 | 1.0671 | 0.3622 | 0.022* | |
O2F | 0.03402 (7) | 0.46140 (10) | 0.45049 (5) | 0.00979 (11) | |
H2FA | 0.0302 | 0.3855 | 0.4041 | 0.015* | |
O3F | 0.33526 (7) | 0.41869 (11) | 0.49451 (5) | 0.01196 (12) | |
H3FA | 0.2829 | 0.3267 | 0.5004 | 0.018* | |
O4F | 0.39729 (7) | 0.80024 (11) | 0.49324 (5) | 0.01022 (11) | |
H4FA | 0.4823 | 0.8340 | 0.5024 | 0.015* | |
C1G | −0.32791 (9) | 0.80214 (14) | 0.11858 (6) | 0.00982 (14) | |
H1G | −0.3219 | 0.9420 | 0.1219 | 0.012* | |
C2G | −0.23436 (8) | 0.71631 (13) | 0.20716 (6) | 0.00919 (13) | |
H2G | −0.2543 | 0.5790 | 0.2096 | 0.011* | |
C3G | −0.07891 (8) | 0.74567 (12) | 0.20083 (6) | 0.00806 (13) | |
H3G | −0.0559 | 0.8822 | 0.2081 | 0.010* | |
C4G | −0.04891 (8) | 0.67751 (13) | 0.10331 (6) | 0.00803 (13) | |
H4G | −0.0682 | 0.5399 | 0.0971 | 0.010* | |
C5G | −0.14732 (9) | 0.78118 (13) | 0.02294 (6) | 0.00859 (13) | |
H5G | −0.1318 | 0.9192 | 0.0311 | 0.010* | |
O5G | −0.28938 (6) | 0.73757 (11) | 0.03071 (4) | 0.01060 (11) | |
C6G | −0.12642 (9) | 0.72355 (14) | −0.07751 (6) | 0.01059 (14) | |
H6G1 | −0.0240 | 0.7129 | −0.0782 | 0.013* | |
H6G2 | −0.1692 | 0.5985 | −0.0927 | 0.013* | |
O7M | −0.46601 (7) | 0.74456 (12) | 0.11844 (5) | 0.01356 (13) | |
C7M | −0.56733 (10) | 0.8496 (2) | 0.05233 (8) | 0.0228 (2) | |
H71 | −0.5534 | 0.9837 | 0.0658 | 0.034* | |
H72 | −0.6629 | 0.8136 | 0.0605 | 0.034* | |
H73 | −0.5551 | 0.8234 | −0.0145 | 0.034* | |
O2G | −0.27031 (8) | 0.80440 (12) | 0.29016 (5) | 0.01355 (13) | |
H2G1 | −0.2675 | 0.7254 | 0.3350 | 0.020* | |
O3G | 0.01081 (7) | 0.64343 (10) | 0.27542 (4) | 0.00823 (11) | |
O4G | 0.09313 (7) | 0.71213 (11) | 0.09408 (5) | 0.01101 (12) | |
H4G1 | 0.1439 | 0.6219 | 0.1178 | 0.017* | |
O6G | −0.18739 (7) | 0.85281 (11) | −0.15098 (5) | 0.01114 (12) | |
H6G | −0.2758 | 0.8442 | −0.1596 | 0.017* | |
OW1 | −0.23629 (7) | 0.58785 (10) | 0.45089 (5) | 0.01226 (12) | |
H11 | −0.1538 (15) | 0.546 (3) | 0.4560 (12) | 0.018* | |
H12 | −0.2840 (16) | 0.500 (3) | 0.4666 (12) | 0.018* | |
OW2 | 0.02672 (8) | 0.22661 (11) | 0.29518 (5) | 0.01423 (13) | |
H21 | 0.0697 (18) | 0.276 (3) | 0.2567 (12) | 0.021* | |
H22 | 0.0714 (19) | 0.124 (2) | 0.3125 (13) | 0.021* | |
OW3 | 0.53751 (9) | 0.90725 (15) | 0.79144 (8) | 0.02575 (19) | |
H31 | 0.4671 (19) | 0.846 (3) | 0.7728 (15) | 0.039* | |
H32 | 0.511 (2) | 1.000 (3) | 0.8244 (15) | 0.039* | |
OW4A | −0.28298 (13) | 1.20268 (19) | 0.26647 (12) | 0.0277 (4) | 0.748 (4) |
H41A | −0.297 (3) | 1.097 (3) | 0.288 (2) | 0.042* | 0.748 (4) |
H42A | −0.196 (2) | 1.217 (5) | 0.276 (2) | 0.042* | 0.748 (4) |
OW4B | −0.3277 (4) | 1.2108 (6) | 0.3181 (2) | 0.0192 (9) | 0.252 (4) |
H41B | −0.269 (7) | 1.128 (10) | 0.313 (6) | 0.029* | 0.252 (4) |
H42B | −0.307 (7) | 1.261 (10) | 0.371 (3) | 0.029* | 0.252 (4) |
U11 | U22 | U33 | U12 | U13 | U23 | |
C1F | 0.0070 (3) | 0.0093 (3) | 0.0069 (3) | 0.0005 (3) | 0.0005 (2) | 0.0001 (2) |
C2F | 0.0065 (3) | 0.0099 (3) | 0.0070 (3) | 0.0003 (2) | 0.0007 (2) | 0.0009 (2) |
C3F | 0.0061 (3) | 0.0088 (3) | 0.0088 (3) | 0.0007 (2) | 0.0007 (2) | 0.0010 (3) |
C4F | 0.0063 (3) | 0.0097 (3) | 0.0081 (3) | −0.0002 (2) | 0.0009 (2) | 0.0001 (2) |
C5F | 0.0080 (3) | 0.0111 (3) | 0.0090 (3) | 0.0001 (3) | 0.0015 (2) | 0.0014 (3) |
O5F | 0.0076 (2) | 0.0089 (3) | 0.0114 (3) | 0.0000 (2) | 0.00116 (19) | 0.0009 (2) |
C6F | 0.0130 (3) | 0.0144 (4) | 0.0165 (4) | −0.0030 (3) | 0.0028 (3) | 0.0058 (3) |
O2F | 0.0081 (2) | 0.0116 (3) | 0.0100 (2) | −0.0015 (2) | 0.00258 (19) | 0.0013 (2) |
O3F | 0.0076 (2) | 0.0121 (3) | 0.0152 (3) | 0.0007 (2) | −0.0007 (2) | 0.0063 (2) |
O4F | 0.0071 (2) | 0.0134 (3) | 0.0097 (2) | −0.0020 (2) | 0.00025 (18) | −0.0024 (2) |
C1G | 0.0070 (3) | 0.0138 (3) | 0.0086 (3) | 0.0012 (3) | 0.0013 (2) | 0.0021 (3) |
C2G | 0.0078 (3) | 0.0125 (3) | 0.0072 (3) | 0.0013 (3) | 0.0013 (2) | 0.0014 (3) |
C3G | 0.0075 (3) | 0.0095 (3) | 0.0067 (3) | 0.0011 (3) | 0.0000 (2) | 0.0014 (3) |
C4G | 0.0067 (3) | 0.0101 (3) | 0.0071 (3) | 0.0001 (2) | 0.0007 (2) | 0.0007 (2) |
C5G | 0.0080 (3) | 0.0105 (3) | 0.0073 (3) | −0.0002 (2) | 0.0012 (2) | 0.0004 (3) |
O5G | 0.0069 (2) | 0.0165 (3) | 0.0081 (2) | −0.0010 (2) | 0.00048 (18) | −0.0002 (2) |
C6G | 0.0121 (3) | 0.0125 (3) | 0.0070 (3) | 0.0010 (3) | 0.0012 (2) | 0.0007 (3) |
O7M | 0.0058 (2) | 0.0206 (3) | 0.0140 (3) | 0.0013 (2) | 0.0009 (2) | 0.0068 (3) |
C7M | 0.0092 (3) | 0.0371 (6) | 0.0211 (4) | 0.0065 (4) | 0.0002 (3) | 0.0131 (4) |
O2G | 0.0137 (3) | 0.0198 (3) | 0.0079 (2) | 0.0055 (3) | 0.0039 (2) | 0.0018 (2) |
O3G | 0.0086 (2) | 0.0092 (3) | 0.0059 (2) | 0.0017 (2) | −0.00137 (18) | −0.0001 (2) |
O4G | 0.0066 (2) | 0.0156 (3) | 0.0111 (2) | 0.0003 (2) | 0.00206 (19) | 0.0016 (2) |
O6G | 0.0108 (2) | 0.0142 (3) | 0.0076 (2) | −0.0013 (2) | −0.00058 (19) | 0.0023 (2) |
OW1 | 0.0111 (3) | 0.0116 (3) | 0.0148 (3) | 0.0017 (2) | 0.0043 (2) | 0.0029 (2) |
OW2 | 0.0165 (3) | 0.0106 (3) | 0.0163 (3) | 0.0008 (2) | 0.0047 (2) | 0.0010 (2) |
OW3 | 0.0134 (3) | 0.0249 (4) | 0.0370 (5) | 0.0026 (3) | −0.0009 (3) | −0.0141 (4) |
OW4A | 0.0169 (5) | 0.0176 (6) | 0.0475 (9) | 0.0016 (4) | 0.0029 (5) | 0.0056 (5) |
OW4B | 0.0190 (14) | 0.0227 (17) | 0.0162 (14) | 0.0014 (12) | 0.0039 (11) | −0.0007 (12) |
C1F—O3G | 1.4122 (10) | C3G—H3G | 1.0000 |
C1F—O5F | 1.4149 (11) | C4G—O4G | 1.4158 (10) |
C1F—C2F | 1.5292 (11) | C4G—C5G | 1.5265 (11) |
C1F—H1F | 1.0000 | C4G—H4G | 1.0000 |
C2F—O2F | 1.4205 (11) | C5G—O5G | 1.4236 (10) |
C2F—C3F | 1.5261 (11) | C5G—C6G | 1.5094 (12) |
C2F—H2F | 1.0000 | C5G—H5G | 1.0000 |
C3F—O3F | 1.4197 (10) | C6G—O6G | 1.4272 (11) |
C3F—C4F | 1.5182 (12) | C6G—H6G1 | 0.9900 |
C3F—H3F | 1.0000 | C6G—H6G2 | 0.9900 |
C4F—O4F | 1.4240 (11) | O7M—C7M | 1.4311 (12) |
C4F—C5F | 1.5248 (12) | C7M—H71 | 0.9800 |
C4F—H4F | 1.0000 | C7M—H72 | 0.9800 |
C5F—O5F | 1.4479 (11) | C7M—H73 | 0.9800 |
C5F—C6F | 1.5094 (13) | O2G—H2G1 | 0.8400 |
C5F—H5F | 1.0000 | O4G—H4G1 | 0.8400 |
C6F—H6FA | 0.9800 | O6G—H6G | 0.8400 |
C6F—H6FB | 0.9800 | OW1—H11 | 0.839 (14) |
C6F—H6FC | 0.9800 | OW1—H12 | 0.830 (15) |
O2F—H2FA | 0.8400 | OW2—H21 | 0.814 (15) |
O3F—H3FA | 0.8400 | OW2—H22 | 0.862 (15) |
O4F—H4FA | 0.8400 | OW3—H31 | 0.810 (16) |
C1G—O7M | 1.3896 (11) | OW3—H32 | 0.868 (16) |
C1G—O5G | 1.4200 (11) | OW4A—OW4B | 0.904 (4) |
C1G—C2G | 1.5247 (11) | OW4A—H41A | 0.835 (19) |
C1G—H1G | 1.0000 | OW4A—H42A | 0.826 (18) |
C2G—O2G | 1.4143 (11) | OW4A—H41B | 0.83 (9) |
C2G—C3G | 1.5271 (11) | OW4B—H41A | 0.98 (3) |
C2G—H2G | 1.0000 | OW4B—H41B | 0.83 (2) |
C3G—O3G | 1.4306 (10) | OW4B—H42B | 0.81 (2) |
C3G—C4G | 1.5213 (11) | ||
O3G—C1F—O5F | 112.20 (6) | O2G—C2G—C1G | 107.01 (7) |
O3G—C1F—C2F | 107.64 (7) | O2G—C2G—C3G | 111.67 (7) |
O5F—C1F—C2F | 110.98 (6) | C1G—C2G—C3G | 109.98 (7) |
O3G—C1F—H1F | 108.6 | O2G—C2G—H2G | 109.4 |
O5F—C1F—H1F | 108.6 | C1G—C2G—H2G | 109.4 |
C2F—C1F—H1F | 108.6 | C3G—C2G—H2G | 109.4 |
O2F—C2F—C3F | 111.59 (7) | O3G—C3G—C4G | 107.73 (7) |
O2F—C2F—C1F | 111.36 (6) | O3G—C3G—C2G | 111.03 (7) |
C3F—C2F—C1F | 111.08 (7) | C4G—C3G—C2G | 110.46 (7) |
O2F—C2F—H2F | 107.5 | O3G—C3G—H3G | 109.2 |
C3F—C2F—H2F | 107.5 | C4G—C3G—H3G | 109.2 |
C1F—C2F—H2F | 107.5 | C2G—C3G—H3G | 109.2 |
O3F—C3F—C4F | 109.32 (6) | O4G—C4G—C3G | 111.31 (7) |
O3F—C3F—C2F | 110.63 (7) | O4G—C4G—C5G | 109.37 (7) |
C4F—C3F—C2F | 109.91 (7) | C3G—C4G—C5G | 108.26 (7) |
O3F—C3F—H3F | 109.0 | O4G—C4G—H4G | 109.3 |
C4F—C3F—H3F | 109.0 | C3G—C4G—H4G | 109.3 |
C2F—C3F—H3F | 109.0 | C5G—C4G—H4G | 109.3 |
O4F—C4F—C3F | 109.40 (7) | O5G—C5G—C6G | 107.31 (7) |
O4F—C4F—C5F | 111.56 (7) | O5G—C5G—C4G | 108.48 (7) |
C3F—C4F—C5F | 108.13 (6) | C6G—C5G—C4G | 112.66 (7) |
O4F—C4F—H4F | 109.2 | O5G—C5G—H5G | 109.4 |
C3F—C4F—H4F | 109.2 | C6G—C5G—H5G | 109.4 |
C5F—C4F—H4F | 109.2 | C4G—C5G—H5G | 109.4 |
O5F—C5F—C6F | 107.09 (7) | C1G—O5G—C5G | 113.24 (6) |
O5F—C5F—C4F | 109.44 (7) | O6G—C6G—C5G | 112.81 (8) |
C6F—C5F—C4F | 113.06 (7) | O6G—C6G—H6G1 | 109.0 |
O5F—C5F—H5F | 109.1 | C5G—C6G—H6G1 | 109.0 |
C6F—C5F—H5F | 109.1 | O6G—C6G—H6G2 | 109.0 |
C4F—C5F—H5F | 109.1 | C5G—C6G—H6G2 | 109.0 |
C1F—O5F—C5F | 115.83 (7) | H6G1—C6G—H6G2 | 107.8 |
C5F—C6F—H6FA | 109.5 | C1G—O7M—C7M | 112.83 (8) |
C5F—C6F—H6FB | 109.5 | O7M—C7M—H71 | 109.5 |
H6FA—C6F—H6FB | 109.5 | O7M—C7M—H72 | 109.5 |
C5F—C6F—H6FC | 109.5 | H71—C7M—H72 | 109.5 |
H6FA—C6F—H6FC | 109.5 | O7M—C7M—H73 | 109.5 |
H6FB—C6F—H6FC | 109.5 | H71—C7M—H73 | 109.5 |
C2F—O2F—H2FA | 109.5 | H72—C7M—H73 | 109.5 |
C3F—O3F—H3FA | 109.5 | C2G—O2G—H2G1 | 109.5 |
C4F—O4F—H4FA | 109.5 | C1F—O3G—C3G | 114.75 (7) |
O7M—C1G—O5G | 107.29 (7) | C4G—O4G—H4G1 | 109.5 |
O7M—C1G—C2G | 108.04 (7) | C6G—O6G—H6G | 109.5 |
O5G—C1G—C2G | 111.41 (7) | H11—OW1—H12 | 105.3 (16) |
O7M—C1G—H1G | 110.0 | H21—OW2—H22 | 105.5 (16) |
O5G—C1G—H1G | 110.0 | H31—OW3—H32 | 106.1 (18) |
C2G—C1G—H1G | 110.0 | ||
O3G—C1F—C2F—O2F | −52.73 (8) | O2G—C2G—C3G—O3G | 69.76 (9) |
O5F—C1F—C2F—O2F | −175.86 (7) | C1G—C2G—C3G—O3G | −171.59 (7) |
O3G—C1F—C2F—C3F | 72.31 (8) | O2G—C2G—C3G—C4G | −170.80 (7) |
O5F—C1F—C2F—C3F | −50.82 (9) | C1G—C2G—C3G—C4G | −52.15 (10) |
O2F—C2F—C3F—O3F | −59.67 (9) | O3G—C3G—C4G—O4G | −61.52 (9) |
C1F—C2F—C3F—O3F | 175.42 (7) | C2G—C3G—C4G—O4G | 177.06 (7) |
O2F—C2F—C3F—C4F | 179.51 (6) | O3G—C3G—C4G—C5G | 178.25 (7) |
C1F—C2F—C3F—C4F | 54.60 (9) | C2G—C3G—C4G—C5G | 56.82 (9) |
O3F—C3F—C4F—O4F | −58.47 (8) | O4G—C4G—C5G—O5G | 177.31 (7) |
C2F—C3F—C4F—O4F | 63.14 (8) | C3G—C4G—C5G—O5G | −61.25 (9) |
O3F—C3F—C4F—C5F | 179.85 (7) | O4G—C4G—C5G—C6G | 58.65 (10) |
C2F—C3F—C4F—C5F | −58.54 (8) | C3G—C4G—C5G—C6G | −179.91 (7) |
O4F—C4F—C5F—O5F | −61.09 (9) | O7M—C1G—O5G—C5G | −177.77 (7) |
C3F—C4F—C5F—O5F | 59.25 (9) | C2G—C1G—O5G—C5G | −59.71 (10) |
O4F—C4F—C5F—C6F | 58.17 (9) | C6G—C5G—O5G—C1G | −173.98 (7) |
C3F—C4F—C5F—C6F | 178.52 (7) | C4G—C5G—O5G—C1G | 64.03 (9) |
O3G—C1F—O5F—C5F | −65.99 (9) | O5G—C5G—C6G—O6G | 77.02 (9) |
C2F—C1F—O5F—C5F | 54.48 (9) | C4G—C5G—C6G—O6G | −163.63 (7) |
C6F—C5F—O5F—C1F | 177.74 (7) | O5G—C1G—O7M—C7M | −73.36 (11) |
C4F—C5F—O5F—C1F | −59.37 (9) | C2G—C1G—O7M—C7M | 166.41 (9) |
O7M—C1G—C2G—O2G | −68.77 (9) | O5F—C1F—O3G—C3G | −69.16 (8) |
O5G—C1G—C2G—O2G | 173.62 (7) | C2F—C1F—O3G—C3G | 168.45 (6) |
O7M—C1G—C2G—C3G | 169.75 (8) | C4G—C3G—O3G—C1F | 144.26 (7) |
O5G—C1G—C2G—C3G | 52.15 (10) | C2G—C3G—O3G—C1F | −94.67 (8) |
D—H···A | D—H | H···A | D···A | D—H···A |
O2F—H2FA···OW2 | 0.84 | 1.89 | 2.7326 (10) | 176 |
O3F—H3FA···O2F | 0.84 | 2.56 | 2.8696 (10) | 103 |
O3F—H3FA···OW1i | 0.84 | 1.92 | 2.7049 (10) | 156 |
O4F—H4FA···O3Fii | 0.84 | 1.85 | 2.6836 (10) | 172 |
O6G—H6G···OW3iii | 0.84 | 1.86 | 2.6546 (11) | 157 |
O2G—H2G1···OW1 | 0.84 | 1.87 | 2.6981 (10) | 168 |
O4G—H4G1···O6Giv | 0.84 | 2.00 | 2.7884 (11) | 155 |
OW1—H11···O2F | 0.84 (2) | 1.92 (2) | 2.7522 (10) | 172 (2) |
OW1—H12···O4Fi | 0.83 (2) | 1.94 (2) | 2.7646 (10) | 178 (2) |
OW2—H21···O6Giv | 0.81 (2) | 2.09 (2) | 2.8897 (10) | 170 (2) |
OW2—H22···O5Fv | 0.86 (2) | 1.91 (2) | 2.7682 (11) | 176 (2) |
OW3—H31···OW4Ai | 0.81 (2) | 2.04 (2) | 2.8424 (16) | 176 (2) |
OW3—H32···O7Mvi | 0.87 (2) | 2.00 (2) | 2.8559 (13) | 169 (2) |
OW4A—H41A···O2G | 0.83 (2) | 2.10 (2) | 2.8615 (16) | 151 (3) |
OW4A—H42A···OW2vii | 0.83 (2) | 2.11 (2) | 2.9390 (15) | 174 (4) |
Symmetry codes: (i) −x, y−1/2, −z+1; (ii) −x+1, y+1/2, −z+1; (iii) x−1, y, z−1; (iv) −x, y−1/2, −z; (v) x, y−1, z; (vi) −x, y+1/2, −z+1; (vii) x, y+1, z. |
Experimental details
Crystal data | |
Chemical formula | C13H24O10·4H2O |
Mr | 412.39 |
Crystal system, space group | Monoclinic, P21 |
Temperature (K) | 100 |
a, b, c (Å) | 9.6150 (2), 7.1362 (1), 13.9716 (2) |
β (°) | 100.1180 (18) |
V (Å3) | 943.75 (3) |
Z | 2 |
Radiation type | Mo Kα |
µ (mm−1) | 0.13 |
Crystal size (mm) | 0.15 × 0.05 × 0.03 |
Data collection | |
Diffractometer | Oxford Xcalibur 3 diffractometer with Sapphire 3 CCD |
Absorption correction | Multi-scan (CrysAlis RED; Oxford Diffraction, 2008) |
Tmin, Tmax | 0.976, 0.996 |
No. of measured, independent and observed [I > 2σ(I)] reflections | 37858, 4836, 4644 |
Rint | 0.030 |
(sin θ/λ)max (Å−1) | 0.833 |
Refinement | |
R[F2 > 2σ(F2)], wR(F2), S | 0.028, 0.073, 1.08 |
No. of reflections | 4836 |
No. of parameters | 294 |
No. of restraints | 16 |
H-atom treatment | H atoms treated by a mixture of independent and constrained refinement |
Δρmax, Δρmin (e Å−3) | 0.44, −0.26 |
Computer programs: CrysAlis CCD (Oxford Diffraction, 2008), CrysAlis RED (Oxford Diffraction, 2008), SHELXS97 (Sheldrick, 2008), SHELXL97 (Sheldrick, 2008), DIAMOND (Brandenburg, 1999), PLATON (Spek, 2009).
D—H···A | D—H | H···A | D···A | D—H···A |
O2F—H2FA···OW2 | 0.84 | 1.89 | 2.7326 (10) | 176 |
O3F—H3FA···O2F | 0.84 | 2.56 | 2.8696 (10) | 103 |
O3F—H3FA···OW1i | 0.84 | 1.92 | 2.7049 (10) | 156 |
O4F—H4FA···O3Fii | 0.84 | 1.85 | 2.6836 (10) | 172 |
O6G—H6G···OW3iii | 0.84 | 1.86 | 2.6546 (11) | 157 |
O2G—H2G1···OW1 | 0.84 | 1.87 | 2.6981 (10) | 168 |
O4G—H4G1···O6Giv | 0.84 | 2.00 | 2.7884 (11) | 155 |
OW1—H11···O2F | 0.84 (2) | 1.92 (2) | 2.7522 (10) | 172 (2) |
OW1—H12···O4Fi | 0.83 (2) | 1.94 (2) | 2.7646 (10) | 178 (2) |
OW2—H21···O6Giv | 0.81 (2) | 2.09 (2) | 2.8897 (10) | 170 (2) |
OW2—H22···O5Fv | 0.86 (2) | 1.91 (2) | 2.7682 (11) | 176 (2) |
OW3—H31···OW4Ai | 0.81 (2) | 2.04 (2) | 2.8424 (16) | 176 (2) |
OW3—H32···O7Mvi | 0.87 (2) | 2.00 (2) | 2.8559 (13) | 169 (2) |
OW4A—H41A···O2G | 0.83 (2) | 2.10 (2) | 2.8615 (16) | 151 (3) |
OW4A—H42A···OW2vii | 0.83 (2) | 2.11 (2) | 2.9390 (15) | 174 (4) |
Symmetry codes: (i) −x, y−1/2, −z+1; (ii) −x+1, y+1/2, −z+1; (iii) x−1, y, z−1; (iv) −x, y−1/2, −z; (v) x, y−1, z; (vi) −x, y+1/2, −z+1; (vii) x, y+1, z. |
Acknowledgements
This work was supported by a grant from the Swedish Research Council (VR).
References
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This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
Many polysaccharides are built of repeating units of oligosaccharides often having four or five sugar residues in their repeats. They may also carry substituents such as O-acetyl groups and/or pyruvic acids as for the Klebsiella capsular polysaccharides (CPS) termed S-53 and K1, respectively (Jansson et al., 1988; Erbing et al., 1976). The CPS of Klebsiella K1 contains a repeating unit consisting of → 4)-β-D-GlcpA-(2,3-Pyr)-(1→ 4)-α-L-Fucp-(1→ 3)-β-D-Glcp-(1→ (Erbing et al., 1976). More recently the CPS S-53 from Klebsiella pneumoniae ATCC 31488 (Jansson et al., 1988) was investigated, which, in addition, contained O-acetyl groups at positions 2 and 3 of the fucosyl residue. The disaccharide structural element is also present in the CPS produced by the thermophilic cynaobacterium Mastigocladus laminosus (Gloaguen et al., 1999) and the exopolysaccharide from Enterobacter amnigenus (Cescutti et al., 2005). The three-dimensional structure of the title compound, as a methyl glycoside model compound, represents an important part in these polysaccharides and may be used as a suitable starting point in modeling of the polymeric structures.
The major degrees of freedom in an oligosaccharide are the torsion angles ϕH, ψH, and ω. For the title compound (I) the two former are present at the glycosidic α-(1 → 3)-linkage. Furthermore, for the glucose residue the ϕH torsion angle is also important. The ω torsion angle describes the conformation of the hydroxymethyl group in the glucose residue. In the title compound both of the ϕH torsion angles in the structure are described by the exo-anomeric conformation with ϕH = 51.1 (1)° for the fucose residue and ϕH = 45.3 (1)° for the glucose residue (Fig. 1). The torsion angle conformation of ψH = 25.8 (1)°.
The conformation of the hydroxymethyl group is described by one of the three rotamers, gauche-trans, gauche-gauche, or trans-gauche with respect to the conformation of C6–O6 to C5–O5 and to C5–C4, respectively. In the present case the glucose residue has the gt conformation with ω = 77.02 (9)°, i.e., shifted away somewhat from a canonical gauche conformation. Extensive water-water hydrogen bonding was observed (Table 1) for the four water molecules present in the crystal. Partial occupancy was observed for one of the water molecules, in a 3:1 relative ratio between OW4A and OW4B, with a distance of 0.90 Å in between the disordered water molecules.
The calculated Cremer & Pople (1975) parameters for the two different rings are: ring O5f → C5f [Q=0.5682 (9) Å, θ=172.92 (9) ° and ϕ=58.2 (7) °], ring O5g → C5g [Q=0.5822 (9) Å, θ=6.17 (9) ° and ϕ=279.5 (8) °]; consequently, the conformation of both rings can be described as chair-forms.
The crystal structure of methyl 3-O-α-L-fucopyranosyl α-D-galactopyranoside was recently determined (Eriksson & Widmalm, 2012), but in contrast to the title compound it did not crystallize as a hydrate. Two stereochemical differences are present between the compounds: firstly at the reducing end where the O-methyl group is located equatorially (β-configuration) in the title compound but axially (α-configuration) in methyl 3-O-α-L-fucopyranosyl α-D-galactopyranoside; secondly, and more interesting, the configuration at the C4 atom of the hexopyranose residue is different, equatorial in the title compound but axial in the previously investigated disaccharide, i.e., from gluco- to galacto-configuration. For the ϕH dihedral angle the conformation at the glycosidic linkage is almost identical with ϕH = 51° herein and ϕH = 55° in methyl 3-O-α-L-fucopyranosyl α-D-galactopyranoside. However, the conformation at the ψH dihedral angle differs significantly with ψH = 26° herein, compared to ψH = -24° in the previously determined compound, i.e., a difference of 50°. Whether the difference of ca 25° from an eclipsed conformation at the ψH dihedral angle represents an intrinsic difference at the glycosidic linkage between 3-O-substituted glucose and galactose residues, or is just due to packing/hydration effects, remains to be elucidated. We note that in water solution the 13C NMR glycosylation shifts (ΔδC), i.e., differences in chemical shifts between the disaccharide and its constituent monosaccharides, for both the C1 and C3 atoms at the glycosidic linkage are lower by ca 1 p.p.m. in the title compound (ΔδC ~7.2 p.p.m.) compared to those in methyl 3-O-α-L-fucopyranosyl α-D-galactopyranoside (ΔδC ~8.3 p.p.m.) (Baumann et al., 1988). These 13C NMR chemical shift differences may be related to different conformational preferences at the ψH dihedral angle.