 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](za5112fig2mag.jpg)
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Figure 2
Crystallization of HemS from Y. enterocolitica. (a) Needle clusters observed in the `hit' condition identified from the high-throughput screening of 864 conditions in sitting-drop vapour-diffusion experiments: 0.1 M MES, 2.0 M ammonium sulfate, 5% PEG 400 pH 6.5. (b) Improved conditions in hanging drops: 0.1 M MES pH 6.5, 1.8 M ammonium sulfate and 4% PEG 400. (c) and (d) Crystals obtained through further optimization in hanging drops in 0.1 M Tris–HCl pH 8.5, 1.8 M ammonium sulfate, 2% PEG 400; drop size was 2 + 2 µl and protein concentration 30 mg ml−1 (bar indicates 100 µm). |
![[Figure 2]](za5112fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
