Figure 2
Multiple sequence alignment showing the sequence conservation in four different regions for PdxK from sheep, E. coli and B. subtilis and E. coli ThiD. Identical residues across all sequences are shown in reverse type on a dark background. Strongly conserved residues are shown in bold. Residues that have been identified from previous structural studies on sheep and E. coli PdxK to form interactions with the substrate are highlighted by grey boxes and can be seen to be different in B. subtilis PdxK, where they are more similar to E. coli ThiD. The sequences were aligned using ClustalW (Thompson et al., 1994) and the figure was prepared using ESPript (Gouet et al., 1999). |