Crystallization and preliminary diffraction studies of prostaglandin E2-specific monoclonal antibody Fab fragment in the ligand complex

Kurahashi, Y.; Sugahara, M.; Ago, H.; Aoyama, S.; Takahashi, N.; Takio, K.; Katsukawa, M.; Yamamoto, S.; Miyano, M.

doi:10.1107/S174430910803131X

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 3
SDS–PAGE (12.5%) of the purified monoclonal anti-PGE₂ antibody (lane 1), Fc fragment (lane 2) and Fab fragment (lane 3). Lane M, molecular-weight markers (labelled in kDa). The samples were prepared prior to electrophoresis by heating in the presence of 1 mM 2-mercaptoethanol.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 64| Part 11| October 2008| Pages 1027-1030

doi:10.1107/S174430910803131X

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