Structure of the ligand-binding domain of rat VDR in complex with the nonsecosteroidal vitamin D₃ analogue YR301

The crystal structure of the ligand-binding domain of rat VDR in complex with a nonsecosteroidal vitamin D₃ analogue YR301 has been determined at 2.0 Å resolution.

Crystallization and X-ray diffraction analysis of N-terminally truncated human ALG-2

Ca²⁺-bound and Zn²⁺-bound forms of ALG-2 were crystallized and X-ray diffraction data were collected to 3.1 and 3.3 Å resolution, respectively.

Rv0802c from Mycobacterium tuberculosis: the first structure of a succinyltransferase with the GNAT fold

The structure of Rv0802c was determined in an unliganded form to 2.0 Å resolution utilizing single-wavelength anomalous dispersion from a samarium soak that resulted in a single bound Sm³⁺:citrate² complex.

Structure of Drosophila Mad MH2 domain

The crystal structure of the MH2 domain of Mad, which closely matches those of other Smad MH2 domains, is reported at 3.2 Å resolution.

Phoenito experiments: combining the strengths of commercial crystallization automation

This paper describes how four general optimization techniques, growth-rate modulation, fine screening, seeding and additive screening, have been adapted for automation in a medium-throughput crystallization service facility.

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of 2-keto-3-de­oxy-6-phosphogluconate (KDPG) aldolase from Streptococcus suis serotype 2

2-Keto-3-deoxy-6-phosphogluconate aldolase from pathogenic S. suis, an attractive drug target, was cloned, overexpressed, purified and crystallized.

Crystallization and preliminary X-ray analysis of the O-carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides

Crystals of NovN, an O-carbamoyltransferase from S. spheroides, were obtained in monoclinic and orthorhombic forms and native X-ray data were recorded to a maximum of 2.3 Å resolution.

Crystallization and preliminary neutron diffraction studies of HIV-1 protease cocrystallized with inhibitor KNI-272

In order to determine the protonation states of the residues within the active site of an HIV-1 protease–inhibitor complex, a crystal of HIV-1 protease complexed with inhibitor (KNI-272) was grown to a size of 1.4 mm³ for neutron diffraction study. The crystal diffracted to 2.3 Å resolution with sufficient quality for further structure determination.

Crystallization and preliminary crystallographic analysis of exo-α-1,5-L-arabino­furanosidase from Streptomyces avermitilis NBRC14893

Exo-α-1,5-L-arabinofuranosidase from S. avermitilis NBRC14893 was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 2.2 Å and belonged to space group P2₁2₁2₁.

Purification, crystallization and preliminary X-ray analysis of adenylylsulfate reductase from Desulfovibrio vulgaris Miyazaki F

The crystallization of adenylylsulfate reductase isolated from the sulfate-reducing bacterium D. vulgaris Miyazaki F is described. The crystals diffracted to 1.7 Å resolution.

Crystallization and preliminary characterization of di­hydropteridine reductase from Dictyostelium discoideum

The dihydropteridine reductase from D. discoideum has been crystallized. Diffraction data were collected from a rectangular-shaped crystal to 2.16 Å resolution.

Expression, crystallization and preliminary X-ray crystallographic analysis of Xoo2316, a predicted 6-phosphogluconolactonase, from Xanthomonas oryzae pv. oryzae

Xoo2316 from X. oryzae pv. oryzae, a predicted 6-phosphogluconolactonase, the second enzyme of the pentose phosphate pathway, has been cloned, purified and crystallized. A native data set was collected to 2.4 Å resolution and a MAD data set was collected to 2.5 Å resolution.

Crystallization and preliminary X-ray analysis of the human respiratory syncytial virus nucleocapsid protein

Crystals of the nucleocapsid protein of human respiratory syncytial virus have been obtained in two forms, the better of which diffracted to 3.6 Å resolution and contained ten subunits in the asymmetric unit.

Crystallization and preliminary X-ray analysis of isomaltase from Saccharomyces cerevisiae

The crystallization and preliminary X-ray analysis of isomaltase is reported.

Crystallization and preliminary diffraction studies of prostaglandin E₂-specific monoclonal antibody Fab fragment in the ligand complex

The Fab fragment of a monoclonal anti-prostaglandin E₂ antibody was prepared and its complex with prostaglandin E₂ was crystallized.

Expression, crystallization and preliminary X-ray crystallographic analysis of peptide de­formylase from Xanthomonas oryzae pv. oryzae

Peptide deformylase (PDF) catalyzes the removal of the N-formyl group from the N-terminus of nascent polypeptides. This process is essential for the survival of bacteria. As it is an antibacterial drug target, PDF from X. oryzae pv. oryzae was cloned, purified and crystallized for preliminary X-ray studies.

Crystallization of Doc and the Phd–Doc toxin–antitoxin complex

Crystals of bacteriophage P1 Doc were grown in the free state, in complex with a 22-amino-acid C-terminal peptide of Phd and in complex with full-length Phd.

Crystallographic characterization of the membrane-targeting domains of the Rac-specific guanine nucleotide-exchange factors Tiam1 and Tiam2

The membrane-targeting domains of the Rac-specific guanine nucleotide-exchange factors Tiam1 and Tiam2 were purified and crystallized.

Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from the extremophilic microorganism Bacillus thermocatenulatus

BTL2, a thermostable enantioselective biocatalyst of interest for industrial applications, has been crystallized using the sitting-drop vapour-diffusion method. Preliminary X-ray data to 2.2 Å resolution allowed the determination of its unit-cell parameters and space group and initiation of its structure determination.

Crystallization of the coiled-coil domain of Atg16 essential for autophagy

The coiled-coil domain of Atg16 was expressed, purified and crystallized. The resultant crystal diffracted to 2.5 Å resolution.

Crystallization and preliminary X-ray diffraction analysis of PAT, an acetyltransferase from Sulfolobus solfataricus

PAT, an acetyltransferase from the archaeon S. solfataricus that specifically acetylates the chromatin protein Alba, was expressed, purified and crystallized.

Purification, crystallization and data collection of Pectobacterium chrysanthemi AcsD, a type A siderophore synthetase

AcsD, an NRPS-independent siderophore synthetase, has been crystallized.

Purification, crystallization and preliminary X-ray diffraction analysis of the Whirly domain of StWhy2 in complex with single-stranded DNA

The Whirly domain of StWhy2 was crystallized both in the free form and in complex with single-stranded DNA.

Expression, purification, crystallization and preliminary X-ray diffraction analysis of Sagittaria sagittifolia arrowhead protease inhibitor API-A in complex with bovine trypsin

Arrowhead protease inhibitor A, a member of the serine protease inhibitor family, from S. sagittifolia has been cloned, expressed, purified and crystallized in complex with bovine trypsin.

Expression, purification and crystallization of the ecto-enzymatic domain of rat E-NTPDase1 CD39

The ecto-enzymatic domain of rat E-NTPDase1 CD39 was expressed and purified and diffraction-quality crystals of the enzyme were obtained.

Purification, crystallization and X-ray diffraction analysis of pavine N-methyltransferase from Thalictrum flavum

The plant enzyme pavine N-methyltransferase from T. flavum has been produced in E. coli, purified and crystallized and its structure has been solved.

Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45γ

The human Gadd45γ protein has been crystallized as a prelude towards determination of its three-dimensional structure by X-ray crystallography.

Crystallization and preliminary X-ray structural studies of adeno-associated virus serotype 6

Adeno-associated virus type 6, a human DNA virus that is being developed as a vector for gene therapy, has been crystallized in a form suitable for structure determination at about 3.2 Å resolution.

Crystallization and preliminary X-ray analysis of the NKX2.5 homeodomain in complex with DNA

The NKX2.5 homeodomain has been crystallized in complex with DNA. Diffraction data were collected to 1.7 Å resolution.

Expression, purification, crystallization and preliminary X-ray studies of the TAN1 orthologue from Methanothermobacter thermautotrophicus

MTH909, the M. thermautotrophicus orthologue of S. cerevisiae TAN1, has been overexpressed, purified and crystallized. X-ray data were collected to 2.85 Å resolution from a crystal belonging to space group P6₁22 (or P6₅22).

Purification, crystallization and preliminary crystallographic study of the putative enolase MJ0232 from the hyperthermophilic archaeon Methanococcus jannaschii

The putative enolase MJ0232 from M. jannaschii was purified and crystallized. A native data set was collected to 1.85 Å resolution. Preliminary crystallographic analysis and a dynamic light-scattering experiment suggested biological relevance of the octameric state of MJ0232 in solution.