 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](ll5172fig1mag.jpg)
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Figure 1
Crystals of the MEDI545 Fab–rhIFN-α-2a complex. (a) Screening. The crystals shown were obtained during the initial screening phase using Crystal Screen HT (Hampton Research) under condition H9 [0.01 M NiCl2.6H2O, 0.1 M Tris–HCl pH 8.5, 20%(w/v) polyethylene glycol monomethyl ether 2000]. (b) Optimization. The crystals shown grew to dimensions of up to 0.1 × 0.1 × 0.2 mm in hanging drops when 1 µl reservoir solution [200 mM NaI, 8 mM NiCl2.6H2O, 80 mM Tris–HCl pH 8.5, 16%(w/v) polyethylene glycol monomethyl ether 2000] was mixed with 1 µl of a 3.5 mg ml−1 MEDI545 Fab–rhIFN-α-2A solution in 20 mM sodium acetate pH 5.2, 20 mM NaCl. |
![[Figure 1]](ll5172fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
