Crystals on the cover for 2009

The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 Å resolution

The crystal structure of a phosphate-dependent exoribonuclease from B. anthracis has been solved to 1.7 Å resolution. The refined model reveals a hexameric ring structure and five bound sulfate ions per subunit. Each subunit features two sulfate ions located in the active site.

The structure of d(CACACG)·d(CGTGTG)

The crystal structure of d(CACACG)·d(CGTGTG) was solved to a resolution of 2.05 Å in space group P2₁.

Crystallization and preliminary X-ray diffraction analysis of the complex between a human anti-interferon antibody fragment and human interferon α-2A

Crystals of the complex between the Fab fragment of a human anti-interferon α therapeutic antibody and human interferon α-2A have been obtained and diffracted to 3.0 Å resolution.

Crystallization and preliminary X-ray analysis of a C-terminal fragment of FlgJ, a putative flagellar rod cap protein from Salmonella

A C-terminal fragment of Salmonella FlgJ, FlgJ_120–316, which has peptidoglycan-hydrolysing activity, has been overproduced, purified and crystallized and the crystals have been characterized by X-ray diffraction.

Crystallization and preliminary X-ray diffraction analysis of GCIP/HHM transcriptional regulator

Native and selenomethionine-derivatized crystals of full-length human GCIP/HHM protein were obtained. The crystals belonged to space group P3₂21 and the best native crystal diffracted to 3.5 Å resolution.

Crystallization and diffraction patterns of the oxy and cyano forms of the Lucina pectinata haemoglobins complex

The native oxygen-carrier haemoglobins complex (HbII–III) is composed of haemoglobin II (HbII) and haemoglobin III (HbIII), which are found in the ctenidia tissue of the bivalve mollusc Lucina pectinata. This protein complex was isolated and purified from its natural source and crystallized using the vapour-diffusion and capillary counter-diffusion methods.

Overexpression, purification, characterization and preliminary crystallographic study of phosphoglycolate phosphatase from Shigella flexneri 2a strain 301

Recombinant phosphoglycolate phosphatase from S. flexneri was overexpressed, purified, characterized and crystallized using the hanging-drop vapour-diffusion method. SeMet-labelled protein was also prepared and was crystallized for phase determination using the MAD technique.

Crystallization and preliminary crystallographic analysis of cgHle, a homoserine acetyltransferase homologue, from Corynebacterium glutamicum

The enzyme cgHle from C. glutamicum, which has acetyl ester hydrolase activity, was crystallized in four different crystal forms. X-ray diffraction data have been collected to a resolution of 1.2 Å.

Purification, crystallization and preliminary X-ray diffraction analysis of gluta­thionylated Trx1 C33S mutant from yeast

Cytosolic Trx1 containing a C33S mutant was overexpressed, purified, glutathionylated and crystallized. The crystals diffracted to 1.80 Å resolution.

Purification and crystallization of Cor a 9, a major hazelnut allergen

The major hazelnut allergen Cor a 9 was purified from the natural source and crystallized. Diffraction data were collected to 1.9 Å resolution using a synchrotron-radiation source.

Crystallization and preliminary X-ray analysis of FliJ, a cytoplasmic component of the flagellar type III protein-export apparatus from Salmonella sp.

FliJ is one of the essential cytoplasmic components of the flagellar type III protein-export apparatus; it has been expressed, produced and crystallized and the crystals have been characterized by X-ray diffraction.

Cloning, expression, crystallization and preliminary X-ray crystallographic analysis of glutamyl-tRNA synthetase (Xoo1504) from Xanthomonas oryzae pv. oryzae

Glutamyl-tRNA synthetase (GluRS) is an essential enzyme for protein translation and has been considered as a target for drug development. In this paper, the cloning, expression and purification of the GluRS protein encoded by the gltX gene (Xoo1504) and its preliminarily crystallographic analysis are reported.

Cloning, expression, purification, crystallization and preliminary X-ray analysis of the pilus-associated sortase C from Streptococcus pneumoniae

Crystallization conditions and preliminary X-ray diffraction analysis of the S. pneumoniae-derived pilus-associated protein sortase C are reported.

X-ray diffraction analysis of a human tRNA^Gly acceptor-stem microhelix isoacceptor at 1.18 Å resolution

The tRNA^Gly acceptor-stem microhelix isoacceptor from human cytoplasm was crystallized and X-ray diffraction analysis revealed diffraction to 1.18 Å resolution. The sequence of the microhelix was derived from the gene sequence with tRNA Database ID DG9990.

Notes for authors 2009